Impacts of PAR and UV radiation on diurnal photosynthesis performance, pigment composition, and antioxidant function of the hot-spring cyanobacterium Nostoc sp. strain VKB02

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2025-05-12 DOI:10.1007/s00203-025-04338-8

Megha Jaiswal, Nasreen Amin, Vinod K. Kannaujiya

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Abstract

Diurnal oscillations are 24 h clock, that synchronize organisms biological functions based on the daily environmental fluctuation. Continuous increase in ultraviolet radiation have been shown to affect the biological clock of cyanobacteria. The present investigation deals with the 12/12 h of light/dark effects of PAR and PAB (PAR + UVA + UVB) irradiations on ecophysiology and defense management of a hot-spring cyanobacterium Nostoc sp. strain VKB02. The alternative L/D exposure of PAR showed increase in growth and pigment compositions. However, PAB radiation has significantly decreased within the same parameters after the L1 phase except for carotenoid and APC, while PE and PC recovered till the D2 phase corresponding to the counter light phase. The pigments destruction also resulted in Chl a fluorescence (F_v/F_m, F_v׳/F_m׳, Y(II), rETR) emission decline. In addition, PAB exposure accelerated free radicals generation with induced protein oxidation (RCG) and antioxidative enzymes (SOD, POD, CAT) as counteract defense during the light phase. The overall circadian regime facilitated the resynthesis fate of pigment-protein complexes and the mitigation of the high level of ROS production. This result suggests the unique survival strategy of the hot-spring cyanobacterium against ultraviolet radiation in a diurnal manner. This study also offers a deep understanding of the diurnal eco-physiological and biochemical responses of the cyanobacterium for the advancement in sustainable agricultural production with lower input in variable climate.

Graphical Abstract

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PAR和UV辐射对温泉蓝藻Nostoc sp.菌株VKB02日光合性能、色素组成和抗氧化功能的影响

昼夜振荡是24小时的时钟，它根据每天的环境波动同步生物体的生物功能。持续增加的紫外线辐射已被证明会影响蓝藻的生物钟。本文研究了PAR和PAB （PAR + UVA + UVB）照射12/12 h对温泉蓝藻Nostoc sp.菌株VKB02生态生理和防御管理的影响。PAR的交替L/D暴露显示出生长和色素组成的增加。然而，在相同参数下，除类胡萝卜素和APC外，L1期后PAB辐射显著下降，而PE和PC则恢复到D2期对应的逆光期。色素的破坏还导致Chl - a荧光（Fv/Fm、Fv/Fm变化、Y（II）、rETR）发射下降。此外，PAB暴露加速了自由基的产生，诱导蛋白氧化（RCG）和抗氧化酶（SOD， POD， CAT）在光照期起到中和防御作用。整体昼夜节律制度促进了色素蛋白复合物的再合成命运，并减轻了高水平ROS的产生。这一结果表明，温泉蓝藻在昼夜中抵抗紫外线辐射的独特生存策略。该研究还为深入了解蓝藻的昼夜生态生理生化反应提供了依据，为在可变气候条件下推进低投入可持续农业生产提供了依据。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.