Optimized quercetin-loaded glycerohyalurosome hydrogel: an innovative nanoplatform for enhanced wound healing

IF 3.4 Q2 PHARMACOLOGY & PHARMACY

Future Journal of Pharmaceutical Sciences Pub Date : 2025-05-13 DOI:10.1186/s43094-025-00808-4

Walaa Abualsunun, Amerh Aiad Alahmadi, Bayan A. Eshmawi, Osama A. A. Ahmed, Alaa Sirwi, Mahmoud A. Elfaky, Sarah O. Alreeshi, Ibtisam A. Alzahrani, Ibtihal A. Almutairi, Rumaysaa M. Gurunfula, Omaima N. Elgazayerly, Shaimaa M. Badr-Eldin

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引用次数: 0

Abstract

Background

Lipidic nanovesicular systems have attracted researchers’ interest for more effective cutaneous delivery and topical pharmacological efficacy. Quercetin (QUT), a polyphenolic flavonoid known for its antioxidant and anti-inflammatory activity, suffers from poor solubility and bioavailability. The aim of this research was to develop an optimized hydrogel formulation comprising QUT-loaded hyaluronic acid (HYA)-modified glycerosomes (glycerohyalurosomes, GHEs) for effective wound management. A combination of glycerol (GLY) and HYA is being used to provide flexibility to the vesicles for better delivery through the skin; these compounds have been reported to provide benefits for wound healing.

Results

D-optimal design suggested fifteen formulations of QUT-GHEs which were prepared using a modified thin-film hydration method. Results showed that particle sizes ranged from 162.33 to 478.49 nm and zeta potential from −57.8 to −18.8 mV. Transmission electron microscopy confirmed successful loading of the drug into the vesicles. QUT-GHEs were integrated into hydrogel (QUT-GHE-GEL) using 1.5% hydroxypropyl methylcellulose. The pH of the QUT-GHE-GEL was recorded as 5.9 ± 0.03, which is acceptable in wound healing. In vivo studies performed on Wistar rats showed that QUT-GHE-GEL accelerated the wound-healing process compared to the untreated control and marketed product (MP)-treated groups, where a significantly higher wound contraction was observed. Histopathological examination of wound tissues revealed that QUT-GHE-GEL-treated and MP-treated groups exhibited newly sprouted capillaries and enhanced fibroblast development.

Conclusions

Thus, the suggested QUT-GHE-GEL formulation shows promise for effective wound-healing management. QUT-GHE-GEL enhances wound contraction and fosters tissue regeneration while modulating inflammation. The results indicate that QUT-GHE-GEL proves a prospective therapeutic option for wound care applications.

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优化槲皮素负载甘油透明质体水凝胶：一个创新的纳米平台，增强伤口愈合

脂质纳米囊泡系统因其更有效的皮肤递送和局部药理功效而引起了研究人员的兴趣。槲皮素（QUT）是一种多酚类黄酮，具有抗氧化和抗炎活性，但其溶解度和生物利用度较差。本研究的目的是开发一种优化的水凝胶配方，其中包括qut负载透明质酸（HYA）修饰的甘油小体（甘油透明质小体，GHEs），用于有效的伤口管理。甘油（GLY）和HYA的组合被用来为囊泡提供灵活性，以便更好地通过皮肤输送；据报道，这些化合物对伤口愈合有好处。结果采用改进的薄膜水化法制备了15个QUT-GHEs配方。结果表明，该材料的粒径范围为162.33 ~ 478.49 nm， zeta电位范围为−57.8 ~−18.8 mV。透射电镜证实药物成功装载到囊泡中。用1.5%羟丙基甲基纤维素将QUT-GHEs整合到水凝胶（QUT-GHE-GEL）中。记录qut - gh - gel的pH值为5.9±0.03，在伤口愈合中是可以接受的。在Wistar大鼠身上进行的体内研究表明，与未经治疗的对照组和市售产品（MP）治疗组相比，qut - gh - gel加速了伤口愈合过程，观察到伤口收缩明显增强。伤口组织的组织病理学检查显示，qut - gh - gel处理组和mp处理组毛细血管新生，成纤维细胞发育增强。因此，建议的qut - gh - gel配方有望有效地管理伤口愈合。qut - gh - gel增强伤口收缩，促进组织再生，同时调节炎症。结果表明，qut - gh - gel证明了伤口护理应用的前瞻性治疗选择。

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来源期刊

Future Journal of Pharmaceutical Sciences PHARMACOLOGY & PHARMACY-

自引率

0.00%

发文量

审稿时长

23 weeks

期刊介绍： Future Journal of Pharmaceutical Sciences (FJPS) is the official journal of the Future University in Egypt. It is a peer-reviewed, open access journal which publishes original research articles, review articles and case studies on all aspects of pharmaceutical sciences and technologies, pharmacy practice and related clinical aspects, and pharmacy education. The journal publishes articles covering developments in drug absorption and metabolism, pharmacokinetics and dynamics, drug delivery systems, drug targeting and nano-technology. It also covers development of new systems, methods and techniques in pharmacy education and practice. The scope of the journal also extends to cover advancements in toxicology, cell and molecular biology, biomedical research, clinical and pharmaceutical microbiology, pharmaceutical biotechnology, medicinal chemistry, phytochemistry and nutraceuticals.