Irradiation of Mesenchymal Stem Cells with an Argon Plasma Jet with Various Oxygen Admixtures

IF 0.9 4区物理与天体物理 Q4 PHYSICS, FLUIDS & PLASMAS

Plasma Physics Reports Pub Date : 2025-05-12 DOI:10.1134/S1063780X24602207

A. E. Zakharchenko, P. A. Domnin, E. V. Kalinin, A. G. Grosheva, A. V. Petryakov, M. A. Medvedev, E. A. Fefelova, K. Hajisharifi, E. Heydari, H. Mehdian, E. Robert, A. Stancampiano, S. A. Ermolaeva, Yu. S. Akishev

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Abstract

The results of experiments on the usage of a low-temperature plasma (LTP) jet to activate the nutrient liquid medium αMEM containing mesenchymal stem cells (MSCs) isolated from the bone marrow of Wistar rats are presented. The LTP jet had been created by an axially symmetric barrier discharge with a thin rod electrode located inside a quartz tube along its axis. The tube has been purged with argon at a flow rate of about 25 m/s at the tube output. The conditions under which LTP activation of the αMEM medium can accelerate MSC proliferation have been studied. It turns out that the final effect of the activated liquid medium on cells strongly depends on the purity of argon used to form the plasma jet. A small admixture of oxygen in argon at a level of 700 ppm leads to the formation of active oxygen species in the discharge and in the plasma jet, as well as ozone at a fairly high concentration. Ozone supplied by a jet into a liquid medium dissolves well in it and, as a strong oxidizer, can have a detrimental effect on stem cells. The results on the difference in the composition of active particles in plasma jets in pure argon and in argon with a small admixture of oxygen are presented, as well as the results of microbiological studies on the effect of two types of plasma jets on mesenchymal stem cells.

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不同氧混合氩等离子体射流照射间充质干细胞

本文报道了低温等离子体射流活化Wistar大鼠骨髓间充质干细胞（MSCs）营养液αMEM的实验结果。LTP射流是通过沿轴向放置在石英管内的细棒电极的轴对称阻挡放电而产生的。用氩气在管出口处以约25米/秒的流速吹扫管。研究了LTP活化α - mem培养基加速MSC增殖的条件。结果表明，活化液体介质对细胞的最终影响很大程度上取决于用于形成等离子体射流的氩气的纯度。在700 ppm水平的氩气中加入少量氧气，就会在放电和等离子体射流中形成活性氧，以及相当高浓度的臭氧。臭氧被喷射到液体介质中溶解得很好，作为一种强氧化剂，可以对干细胞产生有害影响。本文介绍了纯氩和含少量氧的氩气等离子体射流中活性粒子组成的差异，以及两种等离子体射流对间充质干细胞影响的微生物学研究结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plasma Physics Reports 物理-物理：流体与等离子体

CiteScore

1.90

自引率

36.40%

发文量

104

审稿时长

4-8 weeks

期刊介绍： Plasma Physics Reports is a peer reviewed journal devoted to plasma physics. The journal covers the following topics: high-temperature plasma physics related to the problem of controlled nuclear fusion based on magnetic and inertial confinement; physics of cosmic plasma, including magnetosphere plasma, sun and stellar plasma, etc.; gas discharge plasma and plasma generated by laser and particle beams. The journal also publishes papers on such related topics as plasma electronics, generation of radiation in plasma, and plasma diagnostics. As well as other original communications, the journal publishes topical reviews and conference proceedings.