Biodentine Counteracts the Aging Process of Human Dental Pulp Stem Cells Through Wnt/β-Catenin Pathway

IF 3.2 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

International dental journal Pub Date : 2025-05-11 DOI:10.1016/j.identj.2025.03.028

Qi Zhang , Shujie Cao , Min Zhang , Daming Wu , Chengfei Zhang , Jin Li

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引用次数: 0

Abstract

Objectives

This study aimed to evaluate the effects of Biodentine on human Dental Pulp Stem Cells (hDPSCs) proliferation and senescence and explore the underlying anti-aging mechanism.

Methods

hDPSCs were isolated from two groups of patients aged 18 to 27 and 60 to 70 years and treated with Biodentine extract. The optimal concentration of Biodentine extract was determined using the CCK-8 assay. Biodentine treatment on hDPSCs proliferation and aging was assessed using qRT-PCR and Western blot. The small molecule inhibitor XAV939, targeting the Wnt/β-catenin signaling pathway, was added to investigate the anti-aging mechanism of Biodentine in hDPSCs.

Results

The isolated hDPSCs exhibited mesenchymal stem cells (MSCs) characteristics, and the proportion of S-phased cells decreased significantly in the aged group. Biodentine promoted hDPSCs proliferation, with the most pronounced effect observed at 0.2 mg/mL. qRT-PCR showed that Biodentine enhanced the expression of odonto/osteogenesis-related genes. Senescence-associated β-galactosidase staining and Western blot analysis further demonstrated the significant anti-aging effect of Biodentine on hDPSCs. Western blot revealed that Biodentine exerted its anti-aging effect through the Wnt/β-catenin signaling pathway.

Conclusion

The study demonstrated that Biodentine promotes proliferation and exerts anti-aging effects on hDPSCs through the Wnt/β-catenin pathway.

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生物牙本质通过Wnt/β-Catenin通路抑制人牙髓干细胞的衰老过程

目的研究生物牙本质素对人牙髓干细胞（hDPSCs）增殖和衰老的影响，探讨其抗衰老机制。方法分别从18 ~ 27岁和60 ~ 70岁两组患者中分离dpscs，并给予百妥定提取物治疗。采用CCK-8法确定生物登汀提取物的最佳浓度。采用qRT-PCR和Western blot检测生物登汀对hDPSCs增殖和衰老的影响。加入靶向Wnt/β-catenin信号通路的小分子抑制剂XAV939，研究Biodentine在hDPSCs中的抗衰老机制。结果分离的hDPSCs具有间充质干细胞（MSCs）特征，年龄组s期细胞比例明显降低。Biodentine促进了hDPSCs的增殖，在0.2 mg/mL时效果最明显。qRT-PCR结果显示，Biodentine增强了牙齿/成骨相关基因的表达。衰老相关β-半乳糖苷酶染色和Western blot分析进一步证实了百登汀对hdpsc的显著抗衰老作用。Western blot结果显示，Biodentine通过Wnt/β-catenin信号通路发挥抗衰老作用。结论本研究表明，Biodentine通过Wnt/β-catenin通路促进hDPSCs增殖并发挥抗衰老作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International dental journal 医学-牙科与口腔外科

CiteScore

4.80

自引率

6.10%

发文量

159

审稿时长

63 days

期刊介绍： The International Dental Journal features peer-reviewed, scientific articles relevant to international oral health issues, as well as practical, informative articles aimed at clinicians.