Authentication of seven dried sea cucumber products using SWATH-MS-based proteomics for peptide biomarker discovery and MRM for verification

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control Pub Date : 2025-05-08 DOI:10.1016/j.foodcont.2025.111414

Hongwei Zhang , Xiaomei Zhang , Zhihong Wang , Zhenxing Wu , Bingxue Jiang , Xue Zhao , Jie Xu , Qiang Cai , Yin Zhu , Lingping Hu

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引用次数: 0

Abstract

Economic incentives maintain high risks of sea cucumber adulteration, making species authentication essential for consumer protection and industry sustainability. This study aimed to explore the applicability of peptide biomarkers in species identification of seven commercial dried sea cucumber products. Comparative proteomic analysis was performed with ultrahigh-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) utilizing data-dependent acquisition (DDA) and sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH) techniques. DDA analysis yielded 10,265 peptides assigned to 585 corresponding proteins within the Holothuroidea proteome database, whereas SWATH analysis was used to filter peptides derived from specimen-unique proteins in terms of detectability. Candidate peptides were further subjected to the best local alignment of sequence tags for protein (BLASTp) and multiple reaction monitoring (MRM) analysis to check biological specificity and discrimination capability. Finally, 13 qualified peptides were selected to develop a routine MRM approach and real-life samples evidenced the applicability of the biomarkers.

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利用SWATH-MS-based蛋白质组学发现肽生物标志物和MRM进行验证，对7种海参干产品进行鉴定

经济激励使海参掺假的风险居高不下，因此对消费者保护和行业可持续发展来说，物种认证至关重要。本研究旨在探讨肽类生物标志物在7种海参干产品物种鉴定中的适用性。比较蛋白质组学分析采用超高效液相色谱-高分辨率质谱（UPLC-HRMS）进行，利用数据依赖采集（DDA）和所有理论片段离子质谱（SWATH）技术的顺序窗口采集。DDA分析在Holothuroidea蛋白质组数据库中获得了10265个肽，分配给585个相应的蛋白质，而SWATH分析用于过滤来自标本独特蛋白质的肽。候选肽进一步进行蛋白质序列标签的最佳局部比对（BLASTp）和多反应监测（MRM）分析，以检查生物特异性和识别能力。最后，选择了13个合格的肽来开发常规的MRM方法，实际样品证明了生物标志物的适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Control 工程技术-食品科技

CiteScore

12.20

自引率

6.70%

发文量

758

审稿时长

33 days

期刊介绍： Food Control is an international journal that provides essential information for those involved in food safety and process control. Food Control covers the below areas that relate to food process control or to food safety of human foods: • Microbial food safety and antimicrobial systems • Mycotoxins • Hazard analysis, HACCP and food safety objectives • Risk assessment, including microbial and chemical hazards • Quality assurance • Good manufacturing practices • Food process systems design and control • Food Packaging technology and materials in contact with foods • Rapid methods of analysis and detection, including sensor technology • Codes of practice, legislation and international harmonization • Consumer issues • Education, training and research needs. The scope of Food Control is comprehensive and includes original research papers, authoritative reviews, short communications, comment articles that report on new developments in food control, and position papers.