Low density of intratumoral M1-macrophage infiltration may correlate with worse prognosis in low-grade early-stage uterine endometrioid carcinoma

IF 1.2 Q3 OBSTETRICS & GYNECOLOGY
Michiko Nagamine , Hiroshi Ogi , Maki Hirai , Ikoi Omatsu , Sanzo Moriwaki , Saya Shibata , Satoru Yasukawa , Kaori Yoriki , Motohiro Kojima , Taisuke Mori , Kyoko Itoh , Eiichi Konishi
{"title":"Low density of intratumoral M1-macrophage infiltration may correlate with worse prognosis in low-grade early-stage uterine endometrioid carcinoma","authors":"Michiko Nagamine ,&nbsp;Hiroshi Ogi ,&nbsp;Maki Hirai ,&nbsp;Ikoi Omatsu ,&nbsp;Sanzo Moriwaki ,&nbsp;Saya Shibata ,&nbsp;Satoru Yasukawa ,&nbsp;Kaori Yoriki ,&nbsp;Motohiro Kojima ,&nbsp;Taisuke Mori ,&nbsp;Kyoko Itoh ,&nbsp;Eiichi Konishi","doi":"10.1016/j.gore.2025.101758","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>Low-grade type 1 endometrial carcinoma generally has a favorable prognosis if diagnosed in the early stage. However, a small proportion of the patients experience recurrence, which becomes a significant clinical challenge. Our objective was to explore the differences in tumor immune microenvironment (TIME) between recurrent and cured low-grade early-stage endometrioid carcinoma cases.</div></div><div><h3>Methods</h3><div>We retrospectively examined the TIME in recurrent (n = 11) and non-recurrent (cured, n = 10) cases of low-grade, early-stage endometrioid carcinoma. Cases treated with preoperative or postoperative chemotherapy or radiotherapy were excluded. Multiplex immunohistochemistry was performed on tissue microarrays constructed from formalin-fixed paraffin-embedded tissue blocks of primary surgical specimens and, when available, recurrent lesions. TIME was evaluated by the densities (cell count/mm<sup>2</sup>) of CD68-positive macrophages, M1 (CD80- or CD86-positive) and M2 (CD206-positive) macrophages, CD15-positive neutrophils, and CD3-positive lymphocytes. Intraluminal areas were evaluated separately. In addition, clusters of foam cells in the stroma were visually examined.</div></div><div><h3>Results</h3><div>Compared with the cured group, the primary tumor of recurrent group demonstrated significantly lower densities of CD68-positive macrophages and M1 macrophage. Moreover, analysis of matched primary versus recurrent lesions in a subset of recurrent cases revealed similar immune cell infiltration profiles, suggesting temporal stability of TIME of recurrent cases. Notably, although foam cell clusters were present in both groups with similar frequencies, M1 macrophages were detected exclusively in the cured group, whereas a small number of M2 macrophages were occasionally present in the recurrent group.</div></div><div><h3>Conclusions</h3><div>These findings underscore the potential prognostic value of an activated, pro-inflammatory immune response in early-stage endometrial carcinoma and warrant further investigation into the mechanisms underlying tumor recurrence.</div></div>","PeriodicalId":12873,"journal":{"name":"Gynecologic Oncology Reports","volume":"59 ","pages":"Article 101758"},"PeriodicalIF":1.2000,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gynecologic Oncology Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352578925000839","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Objective

Low-grade type 1 endometrial carcinoma generally has a favorable prognosis if diagnosed in the early stage. However, a small proportion of the patients experience recurrence, which becomes a significant clinical challenge. Our objective was to explore the differences in tumor immune microenvironment (TIME) between recurrent and cured low-grade early-stage endometrioid carcinoma cases.

Methods

We retrospectively examined the TIME in recurrent (n = 11) and non-recurrent (cured, n = 10) cases of low-grade, early-stage endometrioid carcinoma. Cases treated with preoperative or postoperative chemotherapy or radiotherapy were excluded. Multiplex immunohistochemistry was performed on tissue microarrays constructed from formalin-fixed paraffin-embedded tissue blocks of primary surgical specimens and, when available, recurrent lesions. TIME was evaluated by the densities (cell count/mm2) of CD68-positive macrophages, M1 (CD80- or CD86-positive) and M2 (CD206-positive) macrophages, CD15-positive neutrophils, and CD3-positive lymphocytes. Intraluminal areas were evaluated separately. In addition, clusters of foam cells in the stroma were visually examined.

Results

Compared with the cured group, the primary tumor of recurrent group demonstrated significantly lower densities of CD68-positive macrophages and M1 macrophage. Moreover, analysis of matched primary versus recurrent lesions in a subset of recurrent cases revealed similar immune cell infiltration profiles, suggesting temporal stability of TIME of recurrent cases. Notably, although foam cell clusters were present in both groups with similar frequencies, M1 macrophages were detected exclusively in the cured group, whereas a small number of M2 macrophages were occasionally present in the recurrent group.

Conclusions

These findings underscore the potential prognostic value of an activated, pro-inflammatory immune response in early-stage endometrial carcinoma and warrant further investigation into the mechanisms underlying tumor recurrence.
低级别早期子宫内膜样癌肿瘤内浸润低密度的m1巨噬细胞可能与较差的预后相关
目的低级别1型子宫内膜癌早期诊断预后良好。然而,一小部分患者复发,这成为一个重大的临床挑战。我们的目的是探讨复发和治愈的低级别早期子宫内膜样癌患者肿瘤免疫微环境(TIME)的差异。方法回顾性分析低级别早期子宫内膜样癌复发(11例)和未复发(治愈10例)的时间。排除术前或术后化疗或放疗的病例。用福尔马林固定石蜡包埋的组织块构建组织微阵列进行多重免疫组化,这些组织微阵列是由初次手术标本和复发病变(如果有的话)构建的。通过cd68阳性巨噬细胞、M1 (CD80或cd86阳性)和M2 (cd206阳性)巨噬细胞、cd15阳性中性粒细胞和cd3阳性淋巴细胞的密度(细胞计数/mm2)来评估TIME。单独评估腔内区域。此外,视觉检查基质中的泡沫细胞簇。结果与治愈组相比,复发组原发肿瘤中cd68阳性巨噬细胞和M1巨噬细胞密度明显降低。此外,在一组复发病例中,对匹配的原发病变和复发病变的分析显示了相似的免疫细胞浸润谱,这表明复发病例的时间具有时间稳定性。值得注意的是,尽管泡沫细胞团在两组中出现的频率相似,但M1巨噬细胞仅在治愈组中检测到,而少量M2巨噬细胞偶尔出现在复发组中。结论这些发现强调了激活的促炎免疫反应在早期子宫内膜癌中的潜在预后价值,并为进一步研究肿瘤复发的机制提供了依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Gynecologic Oncology Reports
Gynecologic Oncology Reports OBSTETRICS & GYNECOLOGY-
CiteScore
2.00
自引率
0.00%
发文量
183
审稿时长
41 days
期刊介绍: Gynecologic Oncology Reports is an online-only, open access journal devoted to the rapid publication of narrative review articles, survey articles, case reports, case series, letters to the editor regarding previously published manuscripts and other short communications in the field of gynecologic oncology. The journal will consider papers that concern tumors of the female reproductive tract, with originality, quality, and clarity the chief criteria of acceptance.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信