Investigation of an electrical-field-based allergen biosensor utilizing selective polyvinyl chloride membranes for histamine detection

IF 4.1 3区工程技术 Q2 ENGINEERING, ELECTRICAL & ELECTRONIC

Sensors and Actuators A-physical Pub Date : 2025-05-05 DOI:10.1016/j.sna.2025.116603

Yu-Ru Huang , Wei-Cheng Lin , Shang-Chun Chou , Yun-Yu Hsieh , Wei-Lun Yen , Bing-Hong Chen , Chien-Hung Liao

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Abstract

In this study, we developed an electrical-field based biosensor with a Polyvinyl Chloride membrane designed for precise histamine quantification. By employing the lab’s high sensitivity readout circuitry in conjunction with an advanced double cancellation offset comparator, the biosensor demonstrates robust linearity across histamine concentrations ranging from 0.1 μM to 300 μM. The sensor exhibits a sensitivity of 4.2 analog-to-digital (ADC) counts per μM (corresponding to 10.25 mV/μM) and detects a 61.6 % variation in response when histamine interacts with diamine oxidase (DAO). In comparison, the traditional cyclic voltammetry (CV) method shows only a 3.8 % change. This minimal difference underscores the difficulty of accurately distinguishing variations in histamine levels using the CV approach. The proposed biosensor's high sensitivity and specificity to histamine make it well-suited for on-site inspections and portable use in food and fish markets, facilitating early screening and mitigation of histamine-related health risks.

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利用选择性聚氯乙烯膜进行组胺检测的电场过敏原生物传感器的研究

在这项研究中，我们开发了一种基于电场的生物传感器，该传感器带有聚氯乙烯膜，用于精确定量组胺。通过采用实验室的高灵敏度读出电路和先进的双抵消偏移比较器，生物传感器在组胺浓度范围从0.1 μM到300 μM之间表现出强大的线性。该传感器的灵敏度为每μM 4.2个模数（ADC）计数（对应10.25 mV/μM），当组胺与二胺氧化酶（DAO）相互作用时，传感器的响应变化率为61.6 %。相比之下，传统的循环伏安法（CV）仅显示3.8 %的变化。这种微小的差异强调了使用CV方法准确区分组胺水平变化的难度。该生物传感器对组胺的高灵敏度和特异性使其非常适合于现场检查和食品和鱼类市场的便携式使用，有助于早期筛查和减轻组胺相关的健康风险。

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来源期刊

Sensors and Actuators A-physical 工程技术-工程：电子与电气

CiteScore

8.10

自引率

6.50%

发文量

630

审稿时长

49 days

期刊介绍： Sensors and Actuators A: Physical brings together multidisciplinary interests in one journal entirely devoted to disseminating information on all aspects of research and development of solid-state devices for transducing physical signals. Sensors and Actuators A: Physical regularly publishes original papers, letters to the Editors and from time to time invited review articles within the following device areas: • Fundamentals and Physics, such as: classification of effects, physical effects, measurement theory, modelling of sensors, measurement standards, measurement errors, units and constants, time and frequency measurement. Modeling papers should bring new modeling techniques to the field and be supported by experimental results. • Materials and their Processing, such as: piezoelectric materials, polymers, metal oxides, III-V and II-VI semiconductors, thick and thin films, optical glass fibres, amorphous, polycrystalline and monocrystalline silicon. • Optoelectronic sensors, such as: photovoltaic diodes, photoconductors, photodiodes, phototransistors, positron-sensitive photodetectors, optoisolators, photodiode arrays, charge-coupled devices, light-emitting diodes, injection lasers and liquid-crystal displays. • Mechanical sensors, such as: metallic, thin-film and semiconductor strain gauges, diffused silicon pressure sensors, silicon accelerometers, solid-state displacement transducers, piezo junction devices, piezoelectric field-effect transducers (PiFETs), tunnel-diode strain sensors, surface acoustic wave devices, silicon micromechanical switches, solid-state flow meters and electronic flow controllers. Etc...