Genetic parameters and haplotype-based genome-wide association study of indicator traits for gastrointestinal parasite resistance in Santa Ines sheep

IF 2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-05-08 DOI:10.1016/j.vetpar.2025.110498

Karine Assis Costa , André Campêlo Araujo , Pablo Augusto de Souza Fonseca , Hugo Teixeira Silva , Leonardo Sartori Menegatto , Luara Afonso de Freitas , Cleyce Maiara Cardoso , Ivan Carvalho Filho , Pamela Itajara Otto , Ricardo Lopes Dias da Costa , Nedenia Bonvino Stafuzza , Claudia Cristina Paro de Paz

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引用次数: 0

Abstract

Genetic parameters are of great importance in animal breeding since they determine the strategies necessary to increase the genetic progress in economic traits, for example indicator traits for resistance to endoparasites in sheep. Furthermore, genetic markers have been used to identify genomic regions associated with economically important traits, which can help increase the genetic response through genomic selection. Therefore, this study aimed to estimate genetic parameters and perform a haplotype-based genome wide association study (GWAS) to identify genomic regions associated with indicator traits for endoparasite resistance in Santa Ines sheep. Haplotype GWAS was performed using linkage disequilibrium blocks (haploblocks) defined by the Haploview software. Records from 1725 animals for Famacha© (FAM, N = 5560), packed cell volume (PCV, N = 5135), total plasma protein (TPP, N = 4356), and fecal egg count (FEC, N = 4248) were used for analysis. The pedigree file contained information from 4821 animals; of these, 638 animals were genotyped using the Ovine SNP50 Genotyping BeadChip. Heritability estimates were moderate for FAM (0.26), PCV (0.26), TPP (0.16), and log-transformed FEC (0.19). Genomic regions that explained more than 0.3 % of the total additive genetic variance of the traits were defined as significant. These regions overlapped with quantitative traits loci associated with eosinophil number, fecal egg count, hematocrit, and immunoglobulin levels. In the top regions for the traits evaluated we found genes individually involved in inflammatory response, immunity, macrophage function in host-pathogen interactions, and other biological functions. All indicator traits for resistance to gastrointestinal parasites evaluated in this study exhibited sufficient genetic variability to respond to selection and can be used to improve the health and consequently the production of Santa Ines sheep.

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圣伊内斯羊胃肠道寄生虫抗性指标性状的遗传参数及单倍型全基因组关联研究

遗传参数在动物育种中非常重要，因为它们决定了提高经济性状遗传进展所需的策略，例如绵羊抗内寄生虫的指示性状。此外，遗传标记已被用于鉴定与经济上重要性状相关的基因组区域，这有助于通过基因组选择增加遗传反应。因此，本研究旨在估计遗传参数并进行基于单倍型的基因组广泛关联研究（GWAS），以确定与Santa Ines羊体内寄生虫抗性指标性状相关的基因组区域。单倍型GWAS使用Haploview软件定义的连锁不平衡块（haploblocks）进行。使用1725只动物的Famacha©（FAM, N = 5560）、堆积细胞体积（PCV, N = 5135）、血浆总蛋白（TPP, N = 4356）和粪卵计数（FEC, N = 4248）记录进行分析。谱系文件包含来自4821只动物的信息；其中，638只动物使用羊SNP50基因分型头芯片进行了基因分型。FAM（0.26）、PCV（0.26）、TPP（0.16）和对数转换FEC（0.19）的遗传力估计中等。对性状加性遗传变异解释超过0.3 %的基因组区域被定义为显著。这些区域与嗜酸性粒细胞数量、粪卵数量、红细胞压积和免疫球蛋白水平相关的数量性状位点重叠。在性状评估的顶部区域，我们发现了与炎症反应、免疫、宿主-病原体相互作用中的巨噬细胞功能和其他生物功能相关的基因。本研究评估的所有胃肠道寄生虫抗性指标性状均表现出足够的遗传变异性，以响应选择，并可用于改善圣伊内斯羊的健康状况，从而提高产量。

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.