Highly Sensitive Label-Free Immunosensor based on 5-Sulfosalicylic Acid Doped Poly(2-chloroaniline) for Detection of CA72–4 in Gastric Cancer

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-05-08 DOI:10.1016/j.bioelechem.2025.108997

Natthaporn Thongwattana , Nuttha Ariyasajjamongkol , Napa Parinyanitikul , Manit Nithitanakul , Katesara Phasuksom , Anuvat Sirivat

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Abstract

Gastric cancer (GC) is a leading cause of cancer-related deaths worldwide, highlighting the urgent need for sensitive and cost-effective diagnostic tools. In this work, a label-free electrochemical immunosensor (ECI) based on poly(2-chloroaniline) doped with 5-sulfosalicylic acid (dP2ClAn/SSA) was developed for the detection of cancer antigen 72–4 (CA72–4), a key biomarker for the early GC diagnosis. The incorporation of 3-aminopropyltriethoxysilane (APTES) as a linker agent facilitated the antibody immobilization, improved electron transfer, and promoted stable sensor performance. Detection of CA72–4 using differential pulse voltammetry (DPV) demonstrated a broad detection range of 0.001–500 U/mL, with the calculated limit of detection of 0.0053 U/mL (1 U = 1 ng). The sensor exhibited excellent selectivity, reproducibility, and stability over 28 days of storage. Validation with human serum samples confirmed its accuracy and reliability, with performance comparable to ELISA. With its simplicity, portability, and cost-effectiveness, this ECI represents a promising diagnostic tool for early GC detection and clinical point-of-care applications.

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基于5-磺基水杨酸掺杂聚2-氯苯胺的高灵敏度无标记免疫传感器检测胃癌CA72-4

胃癌（GC）是全球癌症相关死亡的主要原因，因此迫切需要敏感且具有成本效益的诊断工具。本研究开发了一种基于聚（2-氯苯胺）掺杂5-磺基水杨酸（dP2ClAn/SSA）的无标记电化学免疫传感器（ECI），用于检测癌症抗原72-4 (CA72-4)， CA72-4是早期胃癌诊断的关键生物标志物。加入3-氨基丙基三乙氧基硅烷（APTES）作为连接剂有助于抗体固定，改善电子转移，并促进稳定的传感器性能。差分脉冲伏安法（DPV）对CA72-4的检测范围为0.001 ~ 500 U/mL，计算检出限为0.0053 U/mL （1 U = 1 ng）。该传感器在28天的存储时间内表现出优异的选择性、重复性和稳定性。人血清样品验证证实了其准确性和可靠性，其性能与ELISA相当。该ECI具有简单性、可移植性和成本效益，是早期GC检测和临床护理点应用的有前途的诊断工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.