Nitrite impairs bioreactor performance due to decreased replication of Candidatus Brocadia sapporoensis by unbalanced energy allocation

IF 11.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2025-05-10 DOI:10.1016/j.watres.2025.123806

Xuejiao Qiao , Liyu Zhang , Zhiguang Qiu , Yang Wu , Chunfang Deng , Yanni Geng , Yichi Zhang , Yan Yan , Bing Li , Lijuan Zhang , Wei-Qin Zhuang , Ke Yu

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Abstract

The effects of nitrite on anammox activities have been extensively studied. However, the molecular mechanisms of specific microorganisms responding to nitrite in anammox systems remain unexplored. This study investigates how excessive nitrite affects the core metabolisms of AnAOB and symbiotic bacteria, further elucidating the mechanisms by which it regulates microbial growth and nitrogen removal performance. Specifically, the nitrogen removal process in a continuous-flow anammox membrane bioreactor collapsed when the nitrite concentration reached 243 mg N/L. Integrated meta-omics analyses demonstrated that excessive nitrite disrupted the energy metabolism of Ca. Brocadia sapporoensis (AMXB1), reducing the energy available for developing tolerance. Subsequently, it disrupted cell replication by impairing the biosynthesis process of AMXB1, particularly DNA replication and the formation of vital cell structures such as the cell membrane and cell wall, as well as the cellular protection system, leading to the collapse of the anammox system. Additionally, the cross-feeding of amino acids and cofactors between AMXB1 and symbiotic bacteria plays an important role in the recovery of nitrogen removal performance of anammox consortia after nitrite inhibition. The findings provide a novel strategy and direction for improving the tolerance and resilience of anammox consortia in engineered ecosystems.

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亚硝酸盐对生物反应器性能的影响是由于能量分配不平衡导致札幌Candidatus Brocadia sapporoensis复制减少

亚硝酸盐对厌氧氨氧化活性的影响已被广泛研究。然而，厌氧氨氧化系统中特定微生物对亚硝酸盐反应的分子机制仍未被探索。本研究探讨过量亚硝酸盐如何影响AnAOB和共生细菌的核心代谢，进一步阐明其调节微生物生长和脱氮性能的机制。其中，连续流厌氧氨氧化膜生物反应器在亚硝酸盐浓度达到243 mg N/L时，脱氮过程崩溃。综合元组学分析表明，过量的亚硝酸盐破坏了Ca. Brocadia sapporoensis （AMXB1）的能量代谢，减少了产生耐受性的能量。随后，它通过破坏AMXB1的生物合成过程，特别是DNA复制和细胞膜、细胞壁等重要细胞结构的形成以及细胞保护系统，破坏细胞复制，导致厌氧氨氧化系统的崩溃。此外，AMXB1与共生菌之间氨基酸和辅因子的交叉取食对亚硝酸盐抑制后厌氧氨氧化菌联合体脱氮性能的恢复起着重要作用。研究结果为提高工程生态系统厌氧氨氧化菌群的耐受性和恢复力提供了新的策略和方向。

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.