Engineering oleaginous yeast Rhodotorula toruloides for production of alkanes and alkenes

IF 6.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Metabolic engineering Pub Date : 2025-05-07 DOI:10.1016/j.ymben.2025.05.003

Yi Yu , Quan Yuan , Jing Dai , Huimin Zhao , Shuobo Shi

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引用次数: 0

Abstract

Due to limited reserves and excessive carbon emission of fossil fuels, there has been an increasing interest in developing advanced biofuels with high energy density such as alkanes and alkenes. Here we report the design and construction of three heterologous biosynthetic pathways of alkanes and alkenes in oleaginous yeast Rhodotorula toruloides, including the AAR/ADO, UndA/UndB and FAP pathways. The performance of various enzymes from different organisms was evaluated within R. toruloides for each pathway. Various metabolic engineering strategies were used to enhance the production of alkanes and alkenes across all three pathways, including enzyme screening, byproduct elimination, and precursor supply enhancement. Notably, the FAP pathway demonstrated significantly superior performance compared to the AAR/ADO and UndA/UndB pathway. As a result, 1.73 g/L alkanes and alkenes were produced from glucose, and 0.94 g/L alkanes and alkenes were produced from lignocellulosic hydrolysates, representing the highest alkanes and alkenes titers reported in yeast. This work establishes R. toruloides as a promising host for hydrocarbons production from glucose and CO₂-neutral feedstocks and paves the way for further strain and process optimization towards industrial production of alkanes and alkenes.

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用于生产烷烃和烯烃的工程产油酵母。

由于化石燃料储量有限，碳排放过高，开发烷烃、烯烃等高能量密度的先进生物燃料日益受到关注。本文报道了产油酵母红酵母（Rhodotorula toruloides）中烷烃和烯烃的三种异源生物合成途径的设计和构建，包括AAR/ADO、UndA/UndB和FAP途径。研究了来自不同生物的各种酶在圆叶蝉体内对每条途径的作用。利用各种代谢工程策略，包括酶筛选、副产物消除和前体供应增强，通过所有三种途径提高烷烃和烯烃的生产。值得注意的是，与AAR/ADO和UndA/UndB途径相比，FAP途径表现出明显优于AAR/ADO和UndA/UndB途径。结果，从葡萄糖中产生1.73 g/L的烷烃和烯烃，从木质纤维素水解物中产生0.94 g/L的烷烃和烯烃，代表了在酵母中报道的最高的烷烃和烯烃滴度。本研究确定了toruloides作为葡萄糖和二氧化碳中性原料生产碳氢化合物的有前途的宿主，并为进一步的菌株和工艺优化铺平了道路，以实现烷烃和烯烃的工业生产。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolic engineering 工程技术-生物工程与应用微生物

CiteScore

15.60

自引率

6.00%

发文量

140

审稿时长

44 days

期刊介绍： Metabolic Engineering (MBE) is a journal that focuses on publishing original research papers on the directed modulation of metabolic pathways for metabolite overproduction or the enhancement of cellular properties. It welcomes papers that describe the engineering of native pathways and the synthesis of heterologous pathways to convert microorganisms into microbial cell factories. The journal covers experimental, computational, and modeling approaches for understanding metabolic pathways and manipulating them through genetic, media, or environmental means. Effective exploration of metabolic pathways necessitates the use of molecular biology and biochemistry methods, as well as engineering techniques for modeling and data analysis. MBE serves as a platform for interdisciplinary research in fields such as biochemistry, molecular biology, applied microbiology, cellular physiology, cellular nutrition in health and disease, and biochemical engineering. The journal publishes various types of papers, including original research papers and review papers. It is indexed and abstracted in databases such as Scopus, Embase, EMBiology, Current Contents - Life Sciences and Clinical Medicine, Science Citation Index, PubMed/Medline, CAS and Biotechnology Citation Index.