Design, construction and evaluation of an innovative horizontal trickle bed reactor for intensification of pectinase production process

IF 3.8 3区工程技术 Q3 ENERGY & FUELS

Chemical Engineering and Processing - Process Intensification Pub Date : 2025-05-10 DOI:10.1016/j.cep.2025.110343

Kavita Singh, Ganti S. Murthy

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Abstract

An innovative horizontal trickle bed reactor (HTBR) was designed and constructed for pectinase production in solid-state fermentation with provisions of mixing and trickling. The HTBR of horizontal configuration consisted of a newly designed impeller with angled blades that provided efficient mixing in linear and circular motions. The moisture and temperature were controlled with a liquid recirculation system which improved heat and mass transfer and also prevented bed drying and compaction. The HTBR performance was evaluated and compared with packed bed and tray bioreactors. The pectinase yield of 242.9 IU/gds was obtained in HTBR, was 2.7-fold higher than the tray reactor and 1.76-fold higher than the packed bed configuration. The pectinase productivity of HTBR was 2.5 IU/gds/h which was 3.5-fold higher than packed bed and 6.09-fold higher than tray configurations. A mathematical model was developed to describe the kinetics of pectinase production as a function of biomass, substrate, agitation, and trickling rates.

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新型卧式滴流床强化果胶酶生产工艺反应器的设计、建造与评价

设计并构建了一种新型的水平滴流床反应器（HTBR），用于固体发酵生产果胶酶，并提供混合和滴流功能。水平配置的HTBR由一个新设计的带角度叶片的叶轮组成，该叶轮在直线和圆周运动中提供了有效的混合。水分和温度控制与液体再循环系统，提高了传热和传质，也防止床干燥和压实。对HTBR的性能进行了评价，并与填料床和托盘生物反应器进行了比较。HTBR的果胶酶产率为242.9 IU/gds，比托盘反应器高2.7倍，比填充床反应器高1.76倍。HTBR的果胶酶产率为2.5 IU/gds/h，是填料床的3.5倍，是托盘的6.09倍。建立了一个数学模型来描述果胶酶生产的动力学，作为生物量、底物、搅拌和滴速的函数。

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来源期刊

Chemical Engineering and Processing - Process Intensification 工程技术-工程：化工

CiteScore

7.80

自引率

9.30%

发文量

408

审稿时长

49 days

期刊介绍： Chemical Engineering and Processing: Process Intensification is intended for practicing researchers in industry and academia, working in the field of Process Engineering and related to the subject of Process Intensification.Articles published in the Journal demonstrate how novel discoveries, developments and theories in the field of Process Engineering and in particular Process Intensification may be used for analysis and design of innovative equipment and processing methods with substantially improved sustainability, efficiency and environmental performance.