Arginine-functionalized graphene quantum dots@palladium composite modified pencil graphite electrode for electrochemical detection of DNA-mitomycin C interaction

IF 4.3 3区材料科学 Q2 MATERIALS SCIENCE, COATINGS & FILMS

Diamond and Related Materials Pub Date : 2025-05-10 DOI:10.1016/j.diamond.2025.112433

Nuray Denizhan , Selehattin Yilmaz , Dilsat Ozkan-Ariksoysal , Deniz Emre , Ali Bilici

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Abstract

In the present study, modified pencil graphite electrodes were prepared by synthesizing a novel nanocomposite with palladium and arginine-functionalized graphene quantum dots (Arg@GQDs) for the electrochemical monitoring of anticancer compound mitomycin C (MC) and double-stranded DNA (dsDNA) interaction for the first time. The oxidation responses of both guanine in DNA and the MC drug were measured in the same potential scanning range, and the drug-DNA interaction was determined by the differential pulse voltammetry (DPV) method. To improve the performance of the developed system, experimental parameters such as dsDNA and MC concentration and their interaction times were optimized. The surfaces obtained after the modification were characterized using scanning electron microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDX), Transmission Electron Microscopy (TEM), X-ray Diffraction (XRD), Ultraviolet-Visible Spectroscopy (UV–Vis), Fourier Transform Infrared Spectroscopy (FTIR), X-ray photon spectroscopy (XPS), Thermogravimetric analysis (TGA) cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods. The developed nanocomposite-modified electrodes (Arg@GQD@Pd@PGEs) provided higher guanine oxidation signals than PGEs. The limit of detection (LOD) values for dsDNA immobilized on PGE and Arg@GQD@Pd@PGEs were 0.713 pg 50 μL⁻¹ and 0.019 pg 50 μL⁻¹, respectively.

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精氨酸功能化石墨烯量子dots@palladium复合材料修饰铅笔石墨电极电化学检测dna -丝裂霉素C相互作用

在本研究中，通过合成钯和精氨酸功能化石墨烯量子点的新型纳米复合材料（Arg@GQDs），首次制备了修饰铅笔石墨电极，用于抗癌化合物丝裂霉素C （MC）和双链DNA （dsDNA）相互作用的电化学监测。在相同的电位扫描范围内测定了鸟嘌呤在DNA和MC药物中的氧化反应，并采用差分脉冲伏安法（DPV）测定了药物与DNA的相互作用。为了提高系统的性能，对dsDNA和MC浓度等实验参数及其相互作用次数进行了优化。利用扫描电镜（SEM）、能量色散x射线能谱（EDX）、透射电镜（TEM）、x射线衍射（XRD）、紫外可见光谱（UV-Vis）、傅里叶变换红外光谱（FTIR）、x射线光子光谱（XPS）、热重分析（TGA）、循环伏安法（CV）和电化学阻抗谱（EIS）等方法对改性后的表面进行了表征。所开发的纳米复合修饰电极（Arg@GQD@Pd@PGEs）提供比PGEs更高的鸟嘌呤氧化信号。PGE和Arg@GQD@Pd@PGEs固定dsDNA的检出限（LOD）分别为0.713 pg 50 μL−1和0.019 pg 50 μL−1。

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来源期刊

Diamond and Related Materials 工程技术-材料科学：综合

CiteScore

6.00

自引率

14.60%

发文量

702

审稿时长

2.1 months

期刊介绍： DRM is a leading international journal that publishes new fundamental and applied research on all forms of diamond, the integration of diamond with other advanced materials and development of technologies exploiting diamond. The synthesis, characterization and processing of single crystal diamond, polycrystalline films, nanodiamond powders and heterostructures with other advanced materials are encouraged topics for technical and review articles. In addition to diamond, the journal publishes manuscripts on the synthesis, characterization and application of other related materials including diamond-like carbons, carbon nanotubes, graphene, and boron and carbon nitrides. Articles are sought on the chemical functionalization of diamond and related materials as well as their use in electrochemistry, energy storage and conversion, chemical and biological sensing, imaging, thermal management, photonic and quantum applications, electron emission and electronic devices. The International Conference on Diamond and Carbon Materials has evolved into the largest and most well attended forum in the field of diamond, providing a forum to showcase the latest results in the science and technology of diamond and other carbon materials such as carbon nanotubes, graphene, and diamond-like carbon. Run annually in association with Diamond and Related Materials the conference provides junior and established researchers the opportunity to exchange the latest results ranging from fundamental physical and chemical concepts to applied research focusing on the next generation carbon-based devices.