A comparative study on ellagic acid's role in salt tolerance, growth, antioxidant system, photochemistry and nitrogen metabolism in wheat and chickpea

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-05-07 DOI:10.1016/j.plaphy.2025.109979

Fevzi Elbasan , Evren Yildiztugay , Ceyda Ozfidan-Konakci , Mehmet Hamurcu

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Abstract

This study aims to evaluate the effectiveness of ellagic acid (EA) in mitigating stress induced by salt and enhancing the tolerance of wheat (a monocot) and chickpea (a dicot). The experiment included four treatment groups: a control (C), 12.5 μM EA application, 100 mM salt (NaCl-S) exposure, and a combined treatment with 12.5 μM EA and 100 mM salt (S + EA). Key physiological (e.g., photosynthetic efficiency: F_v/F_m, F_v/F_o, F_o/F_m), growth, and biochemical responses, including antioxidant enzyme activities (CAT, SOD, POX, APX, GST, GPX, NOX, GR, MDHAR, DHAR) and nitrogen metabolism enzymes (NR, GS, GOGAT, GDH), were evaluated to determine the role of exogenous EA in mitigating salt stress. The application of EA effectively mitigated salt stress in wheat and chickpea by enhancing the relative growth rate (RGR) and relative water content (RWC). EA reduced oxidative stress markers, lowering H₂O₂ levels by 16 % in wheat and 26 % in chickpea, and decreased TBARS content, particularly in wheat. Photosynthetic efficiency was stabilized, especially in wheat, as evidenced by improved OJIP parameters. Antioxidant enzyme activities (CAT, POX) increased in response to EA, with wheat showing greater activity under stress. EA partially restored nitrogen metabolism, with GS and GOGAT activities improving under combined EA and salt treatments, more prominently in wheat. EA enhanced redox homeostasis, with wheat showing a significant increase in tAsA/DHA (76 %) and GSH/GSSG (8 %) , while chickpea showed no change in tAsA/DHA and a decrease in GSH/GSSG under Salt + EA treatment. Overall, EA enhanced salt tolerance by strengthening antioxidant defenses, improving nitrogen assimilation, and stabilizing photosynthesis, with species-specific differences in response patterns.

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鞣花酸在小麦和鹰嘴豆耐盐性、生长、抗氧化系统、光化学和氮代谢中的比较研究

本研究旨在评价鞣花酸（EA）对小麦和鹰嘴豆（鹰嘴豆）减轻盐胁迫和提高耐盐性的有效性。实验分为4个处理组：对照组(C)、12.5 μM EA处理组、100 mM NaCl-S暴露组、12.5 μM EA + 100 mM盐（S + EA）联合处理组。主要生理指标（如光合效率：Fv/Fm、Fv/Fo、Fo/Fm）、生长和生化反应，包括抗氧化酶活性（CAT、SOD、POX、APX、GST、GPX、NOX、GR、MDHAR、DHAR）和氮代谢酶（NR、GS、GOGAT、GDH），以确定外源EA在缓解盐胁迫中的作用。施用EA能提高小麦和鹰嘴豆的相对生长率和相对含水量，有效缓解盐胁迫。EA降低了氧化应激标志物，使小麦和鹰嘴豆的H2O2水平分别降低了16%和26%，并降低了TBARS含量，尤其是小麦。OJIP参数的改善证明了光合效率的稳定，尤其是小麦。小麦抗氧化酶活性（CAT、POX）对EA的响应增加，且在胁迫下表现出更高的活性。EA能部分恢复氮素代谢，且EA和盐联合处理能提高GS和GOGAT活性，在小麦中表现更为明显。在盐+ EA处理下，小麦的tAsA/DHA和GSH/GSSG含量显著增加（76%），鹰嘴豆的tAsA/DHA含量没有变化，GSH/GSSG含量下降（8%）。总的来说，EA通过增强抗氧化防御、促进氮同化和稳定光合作用来增强盐耐受性，但在响应模式上存在物种差异。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.