Patchouli Ethanol Extract Pogostemon cablin Benth. Against Aging Profiles in Doxorubicin-Induced 3T3-L1 Fibroblast Cell Lines

IF 2.2 Q3 GERIATRICS & GERONTOLOGY

Aging Medicine Pub Date : 2025-04-18 DOI:10.1002/agm2.70014

Teguh Santoso, Astrid Feinisa Khairani, Muhammad Hasan Bashari, Julia Ramadhanti, Enny Rohmawati, Monika Pury Oktora, Hesti Lina Wiraswati

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Abstract

Objectives

This research aimed to investigate the potential of Pogostemon cablin Benth's ethanolic extract (PCEE) as an antiaging agent.

Methods

We treated 3T3-L1 cells induced by doxorubicin with PCEE and assessed them with antiaging markers such as GLB-1 gene and telomere length with polymerase chain reaction (PCR), or reactive oxygen species (ROS) accumulation with 2,7-dichlorofluorescein diacetate (DCFHDA).

Results

This study demonstrates that PCEE can inhibit ROS accumulation, decrease the expression level of the senescence-associated β-galactosidase (SA-β-gal) gene, GLB-1, and maintain telomere length in doxorubicin-induced 3T3-L1 cells.

Conclusion

These studies reveal that PCEE is a promising antiaging agent. An in vivo approach should be conducted for further research.

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广藿香乙醇提取物广藿香阿霉素诱导的3T3-L1成纤维细胞抗衰老谱

目的探讨广藿香乙醇提取物（PCEE）作为抗衰老剂的潜力。方法用PCEE处理阿霉素诱导的3T3-L1细胞，用聚合酶链反应（PCR）检测GLB-1基因和端粒长度，用2,7-二氯荧光素（DCFHDA）检测活性氧（ROS）积累。结果在阿霉素诱导的3T3-L1细胞中，PCEE可抑制ROS积累，降低衰老相关β-半乳糖苷酶（SA-β-gal）基因GLB-1的表达水平，维持端粒长度。结论PCEE是一种很有前途的抗衰老剂。进一步的研究需要在体内进行。

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来源期刊

Aging Medicine Medicine-Geriatrics and Gerontology

CiteScore

4.10

自引率

0.00%

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