Individual and simultaneous determination of doxycycline hyclate in veterinary sample

IF 2.2 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Hawbir Muhammed Khdir, Bnar Mahmoud Ibrahim
{"title":"Individual and simultaneous determination of doxycycline hyclate in veterinary sample","authors":"Hawbir Muhammed Khdir,&nbsp;Bnar Mahmoud Ibrahim","doi":"10.1007/s13738-025-03210-2","DOIUrl":null,"url":null,"abstract":"<div><p>A simple, quick, and accurate derivative spectrophotometric technique was created to determine the individual and simultaneous presence of doxycycline hyclate (DOXH) in veterinary samples. The derivative spectra were analyzed by calculating the derivative values using graphical techniques, which included peak-to-baseline, peak-to-peak, and area under peak. The best relationships between DOXH concentrations and peak amplitudes were observed at 219 nm and 232 nm for 2nd derivative and 226, 239, 267, and 281 nm for 3rd derivative, over the concentration range of 1.0–46 μg/ml for 2nd derivative then 1.0–46 μg/ml for 226, 281 nm and 2.0–46 μg/ml for 239, 267 nm for 3rd derivative. To determine the linear relationship between concentrations and area under peaks, the peak-to-peak area method was applied at certain wavelength intervals for 2nd and 3rd derivatives. The peak area is measured within the wavelength intervals of 213–224 nm and 224–242 nm for the second-order derivative spectra. For the third-order derivative spectra, the peak areas are measured within the following wavelength intervals: 218–233 nm, 233–248 nm, 258–274 nm, and 274–292 nm. It was found that the methods worked well enough, with linearity and LOD values lower than 0.624 and 0.660 μg/mL for figuring out the second- and third-order derivatives of DOXH and 0.287 and 0.951 μg/mL for first-order derivatives simultaneous determination of florfenicol (FF) and DOXH, respectively. The suggested approach was used to determine the amount of doxycycline hyclate in pharmaceutical tablets with a recovery rate of 96.8% to 99.6% with an RSD less than 4.96%. The individual and simultaneous quantification of DOXH in veterinary samples can be accomplished with remarkable effectiveness by using the suggested approaches.</p></div>","PeriodicalId":676,"journal":{"name":"Journal of the Iranian Chemical Society","volume":"22 5","pages":"1097 - 1107"},"PeriodicalIF":2.2000,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Iranian Chemical Society","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s13738-025-03210-2","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

Abstract

A simple, quick, and accurate derivative spectrophotometric technique was created to determine the individual and simultaneous presence of doxycycline hyclate (DOXH) in veterinary samples. The derivative spectra were analyzed by calculating the derivative values using graphical techniques, which included peak-to-baseline, peak-to-peak, and area under peak. The best relationships between DOXH concentrations and peak amplitudes were observed at 219 nm and 232 nm for 2nd derivative and 226, 239, 267, and 281 nm for 3rd derivative, over the concentration range of 1.0–46 μg/ml for 2nd derivative then 1.0–46 μg/ml for 226, 281 nm and 2.0–46 μg/ml for 239, 267 nm for 3rd derivative. To determine the linear relationship between concentrations and area under peaks, the peak-to-peak area method was applied at certain wavelength intervals for 2nd and 3rd derivatives. The peak area is measured within the wavelength intervals of 213–224 nm and 224–242 nm for the second-order derivative spectra. For the third-order derivative spectra, the peak areas are measured within the following wavelength intervals: 218–233 nm, 233–248 nm, 258–274 nm, and 274–292 nm. It was found that the methods worked well enough, with linearity and LOD values lower than 0.624 and 0.660 μg/mL for figuring out the second- and third-order derivatives of DOXH and 0.287 and 0.951 μg/mL for first-order derivatives simultaneous determination of florfenicol (FF) and DOXH, respectively. The suggested approach was used to determine the amount of doxycycline hyclate in pharmaceutical tablets with a recovery rate of 96.8% to 99.6% with an RSD less than 4.96%. The individual and simultaneous quantification of DOXH in veterinary samples can be accomplished with remarkable effectiveness by using the suggested approaches.

Abstract Image

兽医样品中水合强力霉素的单独测定和同时测定
建立了一种简单、快速、准确的导数分光光度法测定兽医样品中水合强力霉素(DOXH)的存在。利用图形化技术计算光谱导数值,包括峰对基线、峰对峰和峰下面积。DOXH浓度与峰幅的关系以二阶导数219、232 nm和三阶导数226、239、267、281 nm为最佳,二阶导数1.0 ~ 46 μg/ml、226、281 nm为1.0 ~ 46 μg/ml,三阶导数239、267 nm为2.0 ~ 46 μg/ml。为了确定浓度与峰下面积之间的线性关系,在一定的波长间隔内对二阶和三阶导数采用峰对峰面积法。二阶导数光谱的峰面积分别在213 ~ 224 nm和224 ~ 242 nm波长范围内测量。对于三阶导数光谱,在218 ~ 233nm、233 ~ 248nm、258 ~ 274nm和274 ~ 292nm波长范围内测量峰面积。结果表明,该方法对DOXH的二阶导数和三阶导数同时测定的线性度和LOD值分别小于0.624和0.660 μg/mL,对氟苯尼考(FF)和DOXH的一阶导数同时测定的LOD值分别小于0.287和0.951 μg/mL。该方法可用于测定盐酸多西环素片剂的含量,回收率为96.8% ~ 99.6%,RSD < 4.96%。使用建议的方法,可以实现兽医样品中DOXH的单独和同时定量,效果显著。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
4.40
自引率
8.30%
发文量
230
审稿时长
5.6 months
期刊介绍: JICS is an international journal covering general fields of chemistry. JICS welcomes high quality original papers in English dealing with experimental, theoretical and applied research related to all branches of chemistry. These include the fields of analytical, inorganic, organic and physical chemistry as well as the chemical biology area. Review articles discussing specific areas of chemistry of current chemical or biological importance are also published. JICS ensures visibility of your research results to a worldwide audience in science. You are kindly invited to submit your manuscript to the Editor-in-Chief or Regional Editor. All contributions in the form of original papers or short communications will be peer reviewed and published free of charge after acceptance.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信