Fabrication of chitosan-coated selenium nanoparticles improved anti-inflammation in the treatment of spinal cord injury by reduced ROS and mitochondrial potential

IF 3.5 3区材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

Journal of Materials Science Pub Date : 2025-05-02 DOI:10.1007/s10853-025-10803-y

Zhaofei Wang, Xiangfei Liu, Guoyun He, Xiang Xu, Yansong Wang

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引用次数: 0

Abstract

In this investigation, we designed and synthesized new chitosan-coated selenium nanoparticles functionalized with a peptide PG-6/PTW protein complex polysaccharide loaded with TPZ/RAP. The particle size and zeta potential of cSeNPs@TPZ/RAP were 71.06 ± 5.63 nm and − 17.1 ± 4.35 mV, respectively. These nanoparticles demonstrated a desirable size distribution and excellent stability. In addition, we investigated the protective impact of cSeNPs@TPZ/RAP on PC12 cell lines cytotoxicity induced by hydrogen peroxide (H₂O₂) and the primary mechanism. Moreover, our study revealed that cSeNPs@TPZ/RAP effectively reduced the excessive generation of reactive oxygen species (ROS) to protect against mitochondrial dysfunction. The impact of cSeNPs@TPZ/RAP on recovering function following SCI was assessed using the Basso–Beattie–Bresnahan (BBB) locomotor scale and inclined plane test. The hematoxylin–eosin staining results further demonstrated that cSeNPs@TPZ/RAP exhibited a neuroprotective effect in rats with spinal cord injury (SCI). The finding indicates that cSeNPs@TPZ/RAP has the potential to be advanced as a highly effective nanomedicine for the treatment of spinal cord injuries.

Graphical abstract

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壳聚糖包被硒纳米颗粒的制备通过降低ROS和线粒体电位改善了脊髓损伤治疗中的抗炎作用

在这项研究中，我们设计并合成了新的壳聚糖包被硒纳米粒子，该纳米粒子被肽PG-6/PTW蛋白复合物多糖负载TPZ/RAP。cSeNPs@TPZ/RAP的粒径和zeta电位分别为71.06±5.63 nm和- 17.1±4.35 mV。这些纳米颗粒表现出理想的尺寸分布和优异的稳定性。此外，我们还研究了cSeNPs@TPZ/RAP对过氧化氢（H2O2）诱导的PC12细胞株细胞毒性的保护作用及其主要机制。此外，我们的研究表明cSeNPs@TPZ/RAP可以有效减少活性氧（ROS）的过量产生，从而防止线粒体功能障碍。采用BBB （Basso-Beattie-Bresnahan）运动量表和斜面测试评估cSeNPs@TPZ/RAP对脊髓损伤后功能恢复的影响。苏木精-伊红染色结果进一步证实cSeNPs@TPZ/RAP对脊髓损伤大鼠具有神经保护作用。这一发现表明cSeNPs@TPZ/RAP有潜力成为一种高效的纳米药物，用于治疗脊髓损伤。图形抽象

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来源期刊

Journal of Materials Science 工程技术-材料科学：综合

CiteScore

7.90

自引率

4.40%

发文量

1297

审稿时长

2.4 months

期刊介绍： The Journal of Materials Science publishes reviews, full-length papers, and short Communications recording original research results on, or techniques for studying the relationship between structure, properties, and uses of materials. The subjects are seen from international and interdisciplinary perspectives covering areas including metals, ceramics, glasses, polymers, electrical materials, composite materials, fibers, nanostructured materials, nanocomposites, and biological and biomedical materials. The Journal of Materials Science is now firmly established as the leading source of primary communication for scientists investigating the structure and properties of all engineering materials.