Defining the biomarkers in anti-MRSA fractions of soil Streptomycetes by multivariate analysis

IF 1.8 3区生物学 Q4 MICROBIOLOGY

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Pub Date : 2025-05-10 DOI:10.1007/s10482-025-02087-8

Mohamed Sebak, Venugopal Mukku, Amal E. Saafan, Walid Bakeer, Sameh Abd-ELGhani, Ahmed O. El-Gendy, RuAngelie Edrada-Ebel

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引用次数: 0

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most alarming antibiotic-resistant pathogens causing nosocomial and community-acquired infections. Actinomycetes, particularly Streptomycetes, have historically been a major source of natural products including anti-MRSA agents. Hence, we designed this study to isolate new anti-MRSA natural products from Streptomycetes aided by liquid chromatography-electrospray ionization-high resolution mass spectrometry (LC-ESI-HRMS)-based metabolomics data analysis and dereplication study to select the best fractions for isolation work. Following this approach, two new natural products, namely, (4-propylphenyl) methane-diazonium (1) and (4E)-6-oxooctadec-4-enoic acid (2) and three known compounds, 8-methyl-5-oxononanoic acid (4), 15-methylhexadecanoic acid (5), and (Z)-14-methylpentadecenoic acid (6) were isolated from two soil Streptomyces sp. Additionally, (9E)-11-hydroxyoctadec-9-enoic acid (3) was recovered for the first time from Streptomycetes. The minimum inhibitory concentration (MIC) of compounds 1, 5, and 6 against MRSA were 128, 64, and 64 µg/mL respectively. The compounds were slightly more active against B. subtilis with MIC values of 64, 64, and 32 µg/mL, respectively. Our research adds to the growing knowledge about the importance of combining sophisticated analytical methods in the chemical profiling of the fractions with the outcomes of the bioassays throughout the subsequent fractionation processes to help isolate new secondary metabolites from natural sources.

Graphical abstract

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通过多变量分析确定土壤链霉菌抗mrsa组分的生物标志物

耐甲氧西林金黄色葡萄球菌（MRSA）是引起医院和社区获得性感染的最令人担忧的抗生素耐药病原体之一。放线菌，特别是链菌，历来是抗mrsa药物等天然产物的主要来源。因此，我们设计了这项研究，通过液相色谱-电喷雾电离-高分辨率质谱（LC-ESI-HRMS）为基础的代谢组学数据分析和重复研究，从链霉菌中分离新的抗mrsa天然产物，以选择最佳的分离组分。在此基础上，从两种土壤链霉菌中分离到2个新的天然产物（4-丙基苯基）甲烷-重氮(1)和(4E)-6-氧十六烯-4-烯酸(2)，以及3个已知化合物8-甲基-5-氧十一烯酸(4)、15-甲基十六烯酸(5)和(Z)-14-甲基五烯酸(6)，并首次从链霉菌中分离到(9E)-11-羟基十八烯酸(3)。化合物1、5、6对MRSA的最小抑制浓度（MIC）分别为128、64、64µg/mL。这些化合物对枯草芽孢杆菌的MIC值分别为64、64和32µg/mL，活性稍强。我们的研究增加了关于在随后的分馏过程中将复杂的分析方法与生物测定结果相结合的重要性的知识，以帮助从天然来源中分离出新的次生代谢物。图形抽象

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来源期刊

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology 生物-微生物学

CiteScore

5.60

自引率

11.50%

发文量

104

审稿时长

3 months

期刊介绍： Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.