Spectral and cytotoxicity studies of hybrid silver nanoparticles in human lymphocytes

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-05-08 DOI:10.1016/j.saa.2025.126360

Bojana B. Laban , Uroš Ralević , Andreja Leskovac , Sandra Petrović , Milovan Stoiljković , Milena Rosić , Ana Vujačić Nikezić

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Abstract

This study used Raman spectroscopy and genotoxicity/cytotoxicity assays to investigate the synthesized hybrid silver nanoparticles (Ag@LM/ICG NPs) and their effects on human lymphocytes. Raman spectroscopy was employed to obtain detailed chemical information on Ag@LM/ICG NPs and biochemical insights into untreated and Ag@LM/ICG NPs-treated lymphocytes. While average Raman spectra of NPs-treated cells showed no visible changes at the single-cell level, Raman mapping revealed a high background of some spectra, suggesting the presence of NPs within cells and/or NPs-induced cell structure alterations. Complementary cytotoxicity and genotoxicity assessments showed that Ag@LM/ICG NPs are non-cytotoxic and non-genotoxic to human lymphocytes at the tested concentration range, with cell viability exceeding 84%. These findings underscore the safety and potential of Ag@LM/ICG NPs as biocompatible agents for biomedical applications.

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混合纳米银在人淋巴细胞中的光谱和细胞毒性研究

本研究采用拉曼光谱和遗传毒性/细胞毒性实验研究了合成的杂交银纳米粒子（Ag@LM/ICG NPs）及其对人淋巴细胞的影响。利用拉曼光谱获得Ag@LM/ICG NPs的详细化学信息以及未经处理和Ag@LM/ICG NPs处理的淋巴细胞的生化信息。虽然NPs处理细胞的平均拉曼光谱在单细胞水平上没有明显变化，但拉曼图谱显示一些光谱的高背景，表明细胞内存在NPs和/或NPs诱导的细胞结构改变。互补细胞毒性和遗传毒性评估表明，Ag@LM/ICG NPs在测试浓度范围内对人淋巴细胞无细胞毒性和无基因毒性，细胞存活率超过84%。这些发现强调了Ag@LM/ICG NPs作为生物医学应用的生物相容性制剂的安全性和潜力。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.