Supercritical fluid CO2 extraction followed by polystyrene/divinylbenzene stationary phase complementary supercritical fluid chromatography and reversed-phase liquid chromatography separation of unsaturated fatty acids

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A Pub Date : 2025-05-07 DOI:10.1016/j.chroma.2025.466029

Yingying Tong, Qilan Wang, Jun Dang

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引用次数: 0

Abstract

Supercritical fluid chromatography (SFC) has emerged as a powerful separation technique with extensive applications in pharmaceutical analysis, environmental monitoring, and food safety testing. In this study, weakly polar unsaturated fatty acids were successfully extracted and separated via a polystyrene/divinylbenzene (PS-DVB) stationary phase complementary supercritical fluid chromatography and reversed-phase liquid chromatography (RPLC) approach. The weakly polar sample was obtained using supercritical fluid extraction (SFE) of Floccularia luteovirens powder. The extracted weakly polar sample was first separated using a PS-DVB stationary phase SFC, yielding 304.7 mg of the target fraction Fr2. Then, the established complementary SFC and RPLC method was applied to the separation and purification of compounds from the target fraction Fr2. Finally, three unsaturated fatty acids with purities exceeding 95 % were successfully prepared. This approach has significant potential for analyzing and purifying weakly polar natural products on the same stationary phase using SFC and RPLC.

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超临界流体CO2萃取-聚苯乙烯/二乙烯基苯固定相互补-超临界液相色谱-反相液相色谱分离不饱和脂肪酸

超临界流体色谱（SFC）是一种强大的分离技术，在药物分析、环境监测和食品安全检测等领域有着广泛的应用。本研究采用聚苯乙烯/二乙烯基苯（PS-DVB）固定相互补超临界液相色谱和反相液相色谱（RPLC）分离弱极性不饱和脂肪酸。采用超临界流体萃取法制备了黄絮粉的弱极性样品。提取的弱极性样品首先用PS-DVB固定相SFC分离，得到304.7 mg的目标组分Fr2。然后，将建立的互补SFC和RPLC方法应用于目标馏分Fr2中化合物的分离纯化。最后，成功制备了3种纯度超过95%的不饱和脂肪酸。该方法在利用SFC和RPLC在同一固定相上分析和纯化弱极性天然产物方面具有重要的潜力。

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来源期刊

Journal of Chromatography A 化学-分析化学

CiteScore

7.90

自引率

14.60%

发文量

742

审稿时长

45 days

期刊介绍： The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.