The modulatory effects of persimmon leaf extract on sleep-related neurotransmitters and its potential hypnotic effects

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2025-05-05 DOI:10.1016/j.fitote.2025.106576

Hui Yu , Xian Zhou , Qin Ru , Anu Antony , Morven Cameron , Yang Liu , Isabella Pregardier Klann , Haibiao Guo , Juan Lin , Deqing Wang , Dennis Chang

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Abstract

Purpose

Persimmon leaf is a traditional herbal medicine with diverse therapeutic applications. This study aimed to explore the effect of persimmon leaf extract (PLE) on the modulation of neurotransmitters involved in sleep regulation and its overall impact on sleep latency and duration.

Methods

The key components of PLE were identified by ultra performance liquid chromatography. The modulatory effects of PLE in sleep and wakefulness-related neurotransmitters were studied in human neuroblastoma SH-SY5Y cells. PLE was also investigated in pentobarbital sodium-induced sleep and para-chlorophenylalanine (PCPA)-induced insomnia models in mice and rats.

Results

PLE induced chloride influx and increased the intracellular production of gamma-aminobutyric acid (GABA), a neurotransmitter crucial for sleep regulation, in SH-SY5Y cells. Furthermore, PLE influenced the cellular expressions of serotonin, dopamine, and adenosine. It increased monoamine oxidase enzyme-A activity and reduced serotonin levels and its metabolites. It induced dopamine biosynthesis and degradation pathways. In the pentobarbital-induced sleep experiment, PLE significantly prolonged total sleep duration and reduced sleep latency in a dose-dependent manner. In the PCPA-induced insomnia model, PLE consistently increased GABA production, and lowered dopamine expression.

Conclusion

PLE exhibited modulatory effects on sleep-related neurotransmitters in vitro, which may also contribute to its hypnotic effects by extending the sleep duration and shortening sleeping latency in vivo.

Abstract Image

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柿叶提取物对睡眠相关神经递质的调节作用及其潜在催眠作用

目的柿叶是一种具有多种治疗用途的传统草药。本研究旨在探讨柿叶提取物（PLE）对参与睡眠调节的神经递质的调节作用及其对睡眠潜伏期和持续时间的整体影响。方法采用超高效液相色谱法对其关键成分进行鉴定。在人神经母细胞瘤SH-SY5Y细胞中研究了PLE对睡眠和觉醒相关神经递质的调节作用。在戊巴比妥钠诱导睡眠和对氯苯丙氨酸（PCPA）诱导的小鼠和大鼠失眠模型中也研究了PLE。结果：在SH-SY5Y细胞中，ple诱导氯离子内流并增加细胞内γ -氨基丁酸（GABA）的产生，GABA是一种对睡眠调节至关重要的神经递质。此外，PLE还影响血清素、多巴胺和腺苷的细胞表达。它增加了单胺氧化酶a的活性，降低了血清素水平及其代谢物。诱导多巴胺的生物合成和降解途径。在戊巴比妥诱导睡眠实验中，PLE显著延长总睡眠时间，减少睡眠潜伏期，且呈剂量依赖性。在pppa诱发的失眠模型中，PLE持续增加GABA的产生，降低多巴胺的表达。结论普洱茶在体外对睡眠相关神经递质具有调节作用，其催眠作用可能与普洱茶延长睡眠时间、缩短睡眠潜伏期有关。

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.