Enhanced biosynthesis of 6-aminocaproic acid in engineered Escherichia coli with artificial protein cage-organized enzymatic cascades

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-05-08 DOI:10.1016/j.biortech.2025.132641

Ruoshi Luo , Lin Hu , Dan Wang , Kaixing Xiao , Xuemei Liu , Yaqi Kang , Qinhong Wang

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引用次数: 0

Abstract

Microbial synthesis of 6-aminocaproic acid (6-ACA), a key nylon-6 monomer, was the focus of this study. Our previous work on 6-ACA biosynthesis using an artificial iterative carbon-chain-extension cycle showed potential, but the impact of intermediates on metabolism remained unresolved. To address this, a bacterial microcompartment (BMC) was engineered in Escherichia coli to encapsulate 6-ACA synthesis enzymes, effectively controlling the release of intermediate products. This intervention led to a 90.85 % increase in cell growth and a final 6-ACA yield increase from 46.76 mg/L to 1.12 g/L in a 1 L fermentor. The redesigned BMC demonstrated potential in regulating cascade enzymatic catalysis, particularly in managing intermediates that could impact enzyme proteins, cause cytotoxicity, or DNA damage in cells. This work highlights the potential of the redesigned BMC in enhancing production by controlling the effects of intermediates on cellular processes.

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利用人工蛋白笼组织酶级联增强工程大肠杆菌6-氨基己酸的生物合成

本研究重点研究了尼龙-6关键单体6-氨基己酸（6-ACA）的微生物合成。我们之前使用人工迭代碳链延伸循环进行6-ACA生物合成的工作显示出潜力，但中间体对代谢的影响仍未解决。为了解决这一问题，在大肠杆菌中设计了一个细菌微室（BMC）来包封6-ACA合成酶，有效地控制了中间产物的释放。该干预导致细胞生长增加90.85%，最终6-ACA产量在1l发酵罐中从46.76 mg/L增加到1.12 g/L。重新设计的BMC在调节级联酶催化方面具有潜力，特别是在管理可能影响酶蛋白、导致细胞毒性或细胞DNA损伤的中间体方面。这项工作强调了重新设计的BMC通过控制中间体对细胞过程的影响来提高生产的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.