Sequencing-Based Detection of Measles in Wastewater: Texas, January 2025.

IF 9.6 1区医学 Q1 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

American journal of public health Pub Date : 2025-05-08 DOI:10.2105/ajph.2025.308146

Sara Javornik Cregeen,Michael J Tisza,Blake Hanson,Marissa Cook,Anil Surathu,Rebecca Schneider,Jingjing Wu,Kirstin Short,Kaavya Domakonda,Loren Hopkins,Matthew C Ross,Joseph F Petrosino,Jennifer Deegan,Lauren B Stadler,Eric Boerwinkle,Anthony Maresso

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引用次数: 0

Abstract

Measles is a potentially deadly viral infection spread via respiratory droplets from infected individuals. Outbreaks occur when vaccine coverage drops below the threshold of herd, or community, immunity. Using a sequencing-based approach, we report the prospective (January 7, 2025) detection of measles in nucleic acid extracts from 2 wastewater treatment plants in Houston, Texas, with a population of more than 218 000 residents. The sequencing data from 2 samples contained 53 unique reads mapping to 11 different regions of the measles virus genome with a 99.4% match to genotype B3. Importantly, no detections were observed from 821 previous samples from the same city spanning nearly 3 years of monitoring. The findings were confirmed using droplet digital polymerase chain reaction. A concomitant investigation identified 2 unvaccinated measles-positive travelers living within the same sewershed as the wastewater detection event. This work suggests that sequencing-based wastewater analysis is valuable as a comprehensive early detection warning system that facilitates more targeted epidemiological investigation. (Am J Public Health. Published online ahead of print May 8, 2025:e1-e5. https://doi.org/10.2105/AJPH.2025.308146).

查看原文本刊更多论文

基于测序的废水中麻疹检测：德克萨斯州，2025年1月。

麻疹是一种潜在的致命病毒感染，通过感染者的呼吸道飞沫传播。当疫苗覆盖率低于群体或社区免疫阈值时，就会发生疫情。使用基于测序的方法，我们报告了一项前瞻性研究（2025年1月7日），在德克萨斯州休斯顿的2个污水处理厂的核酸提取物中检测出麻疹，该地区人口超过21.8万。来自2份样本的测序数据包含53个独特的reads，映射到麻疹病毒基因组的11个不同区域，与基因型B3的匹配度为99.4%。重要的是，在近3年的监测中，来自同一城市的821个先前样本未发现任何检测。用液滴数字聚合酶链反应证实了这一发现。同时进行的一项调查发现，与废水检测事件相同的下水道内居住着2名未接种疫苗的麻疹阳性旅行者。这项工作表明，基于测序的废水分析作为一种全面的早期检测预警系统是有价值的，可以促进更有针对性的流行病学调查。公共卫生。2025年5月8日提前在线发布：e1-e5。https://doi.org/10.2105/AJPH.2025.308146)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American journal of public health 医学-公共卫生、环境卫生与职业卫生

CiteScore

9.50

自引率

3.90%

发文量

1109

审稿时长

2-4 weeks

期刊介绍： The American Journal of Public Health (AJPH) is dedicated to publishing original work in research, research methods, and program evaluation within the field of public health. The journal's mission is to advance public health research, policy, practice, and education.