Blastocyst Cavity Expansion Promotes Cell Polarization During Early Development of Mouse Embryos

IF 1.6 4区医学 Q4 DEVELOPMENTAL BIOLOGY

Birth Defects Research Pub Date : 2025-05-09 DOI:10.1002/bdr2.2484

Zheng Guo, Xinxin Lv, Jianwen Li, Shiping Yue, Jing Du

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引用次数: 0

Abstract

Background

Cell polarization is an important morphological process that is crucial for the formation and function of tissues and organs. The blastocyst cavity expansion is an apparent event during the second cell fate specification in mouse embryos, yet its impact on cell polarization remains unclear. In this study, we investigate the effects of blastocyst cavity expansion on cell polarization.

Methods

The methods of this study involve hyperosmotic treatment or disruption of TE cortical tension by laser ablation, combined with immunofluorescence.

Results

We found that inhibition of the blastocyst cavity expansion through hypertonic treatment or disruption of TE cortical tension by laser ablation suppresses the levels of the ζ isotype of protein kinase C (PKC ζ) which is a member of the atypical PKC subfamily involved in cell polarization. We further found that during the embryonic stages E3.5 to E4.0, the expression of extracellular signal-regulated kinase 1 (ERK1), a key upstream regulator of PKC ζ, is altered in a similar tendency to that of PKC ζ, indicating a potential regulatory function of ERK1 in cell polarization during early development of mouse embryos.

Conclusions

This study reveals the function of the mechanical behavior of embryos in cell polarization of early mammalian embryos. The relationship between cell polarization and blastocyst cavity expansion in early embryonic development provides a new understanding, thereby offering fresh insights for the screening and detection of indicators for normal blastocyst development.

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小鼠胚胎早期发育过程中囊胚腔扩张促进细胞极化

细胞极化是一个重要的形态学过程，对组织器官的形成和功能起着至关重要的作用。囊胚腔扩张是小鼠胚胎第二次细胞命运规范过程中的一个明显事件，但其对细胞极化的影响尚不清楚。在本研究中，我们研究了囊胚腔扩张对细胞极化的影响。方法采用高渗治疗或激光消融联合免疫荧光破坏TE皮质张力。结果我们发现，通过高渗治疗抑制囊胚腔扩张或通过激光消融破坏TE皮质张力抑制蛋白激酶C （PKC ζ）的ζ同型水平，PKC ζ是参与细胞极化的非典型PKC亚家族的成员。我们进一步发现，在胚胎阶段E3.5至E4.0，细胞外信号调节激酶1 （ERK1）的表达，PKC ζ的关键上游调节因子，以与PKC ζ相似的趋势改变，表明ERK1在小鼠胚胎早期发育过程中对细胞极化具有潜在的调节功能。结论本研究揭示了胚胎力学行为在哺乳动物早期胚胎细胞极化中的作用。细胞极化与胚泡腔扩张在早期胚胎发育中的关系提供了新的认识，从而为正常胚泡发育指标的筛选和检测提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Birth Defects Research Medicine-Embryology

CiteScore

3.60

自引率

9.50%

发文量

153

期刊介绍： The journal Birth Defects Research publishes original research and reviews in areas related to the etiology of adverse developmental and reproductive outcome. In particular the journal is devoted to the publication of original scientific research that contributes to the understanding of the biology of embryonic development and the prenatal causative factors and mechanisms leading to adverse pregnancy outcomes, namely structural and functional birth defects, pregnancy loss, postnatal functional defects in the human population, and to the identification of prenatal factors and biological mechanisms that reduce these risks. Adverse reproductive and developmental outcomes may have genetic, environmental, nutritional or epigenetic causes. Accordingly, the journal Birth Defects Research takes an integrated, multidisciplinary approach in its organization and publication strategy. The journal Birth Defects Research contains separate sections for clinical and molecular teratology, developmental and reproductive toxicology, and reviews in developmental biology to acknowledge and accommodate the integrative nature of research in this field. Each section has a dedicated editor who is a leader in his/her field and who has full editorial authority in his/her area.