Entering the era of spatial transcriptomics: opportunities and challenges for pathology

Diagnostic Histopathology Pub Date : 2025-05-01 DOI:10.1016/j.mpdhp.2025.03.001

Anna M Sozanska, Carlo Pescia, Emily Thomas, Daniel J Royston, Rosalin A Cooper

引用次数: 0

Abstract

Recent years have seen the emergence of spatial transcriptomic (ST) platforms that are capable of comprehensive whole-section gene expression profiling of tissues with up to subcellular resolution. ST platforms offer novel insights into tissue microenvironments that define health and disease. This article will introduce the principles of ST and outline the commonly used platforms with their relative strengths and weaknesses. It will also discuss key considerations for experimental design and data analysis workflows. Finally, the importance of pathological oversight of ST workflows will be discussed, including the future potential of ST in clinical practice.

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进入空间转录组学时代：病理学的机遇与挑战

近年来，空间转录组学（ST）平台的出现，能够以亚细胞分辨率对组织进行全面的全切片基因表达谱分析。ST平台为定义健康和疾病的组织微环境提供了新的见解。本文将介绍ST的原理，并概述常用平台及其相对优势和劣势。它还将讨论实验设计和数据分析工作流程的关键考虑因素。最后，将讨论ST工作流程的病理监督的重要性，包括ST在临床实践中的未来潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic Histopathology Medicine-Pathology and Forensic Medicine

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： This monthly review journal aims to provide the practising diagnostic pathologist and trainee pathologist with up-to-date reviews on histopathology and cytology and related technical advances. Each issue contains invited articles on a variety of topics from experts in the field and includes a mini-symposium exploring one subject in greater depth. Articles consist of system-based, disease-based reviews and advances in technology. They update the readers on day-to-day diagnostic work and keep them informed of important new developments. An additional feature is the short section devoted to hypotheses; these have been refereed. There is also a correspondence section.