Quantitative assessment and extraction efficiency of phycocyanin from Arthrospira (Spirulina): Impact of algal biomass quality

Q1 Environmental Science

Bioresource Technology Reports Pub Date : 2025-05-06 DOI:10.1016/j.biteb.2025.102130

Ratana Chaiklahan, Nattayaporn Chirasuwan, Thanyalak Srinorasing, Shewin Attasat

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Abstract

Phycocyanin (PC) is a valuable bioactive pigment widely studied for its extraction and purification from Arthrospira biomass. This study investigated the effects of PC content in algal biomass and phosphate buffer concentrations on extraction efficiency and extract quality. Twelve dried Arthrospira samples were analyzed for PC content, which ranged from 6.46 % to 23.20 % of dry weight. Six samples with varying PC content were extracted using a stirrer mixer at room temperature for 2 h, and the results showed that higher PC content in algal biomass significantly improved extraction yield and purity. Extraction of sample No.11 (19.45 % PC content) using 100 mM phosphate buffer achieved the highest yield (16.27 % of dry weight) and best quality, with a purity ratio of 1.87 and 41.35 % PC content in the extract. Although a 10 mM phosphate buffer initially showed lower extraction efficiency for this sample, a second extraction enhanced PC recovery by 85.61 %, making it a cost-effective option for large-scale PC production.

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节肢螺旋藻藻蓝蛋白的定量评价及提取效率：藻类生物量质量的影响

藻蓝蛋白（Phycocyanin， PC）是一种有价值的生物活性色素，从节螺旋藻生物质中提取和纯化得到了广泛的研究。本研究考察了海藻生物量中PC含量和磷酸盐缓冲液浓度对提取效率和提取品质的影响。对12份节肢螺干标本进行了PC含量分析，其含量范围为干重的6.46% ~ 23.20%。采用搅拌混合器对6个不同PC含量的样品在室温下提取2 h，结果表明，藻类生物质中PC含量越高，提取率和纯度越高。采用100 mM磷酸盐缓冲液提取11号样品（PC含量19.45%），得率最高（干重的16.27%），提取液中PC含量为41.35%，纯度比为1.87。虽然10 mM磷酸盐缓冲液最初对该样品的提取效率较低，但第二次提取可使PC回收率提高85.61%，使其成为大规模PC生产的经济有效选择。

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