Rapid On-Site Histopathological Analysis of Kidney Biopsy With Dynamic Full-Field Optical Coherence Tomography

IF 5.7 2区医学 Q1 UROLOGY & NEPHROLOGY

Kidney International Reports Pub Date : 2025-05-01 DOI:10.1016/j.ekir.2025.01.047

Léa Zuccarelli , Quentin Bernard , Georges Tarris , Laurent Martin , Mathilde Funes de la Vega , Amélie Jacq , Jean-Michel Rebibou , Claire Tinel , Claude Boccara , Olivier Thouvenin , Jean-Marie Chassot , Marion Rabant , Julien Zuber , Christophe Legendre , Jean-Pierre Quenot , Marie-Noëlle Peraldi , Lucile Amrouche , Anne Scemla , Nathalie Chavarot , Dany Anglicheau , Thomas Maldiney

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Abstract

Introduction

Kidney histology preparation requires a multistep process that is usually responsible for delayed results. This study introduces dynamic full-field optical coherence tomography (D-FF-OCT) as a label-free alternative to overcome the limitations of traditional histopathology for on-site kidney pathology assessment.

Methods

Two patient cohorts were considered, with a total of 31 patients included in the study; one cohort involved patients requiring biopsy of transplant kidney, and the other involved patients requiring biopsy of native kidney. The clinical and biological data were prospectively collected. Histopathological analysis of kidney biopsies was conducted using both conventional stains and dynamic D-FF-OCT imaging.

Results

D-FF-OCT enabled the recognition of most kidney structures. The results showed a significant correlation between this technology and conventional stains for the evaluation of both interstitial fibrosis (IF) (r = 0.61, P < 0.001) and tubular atrophy (TA) (r = 0.60, P < 0.001). Although many lesions could be identified such as interstitial inflammation, acute tubular necrosis, glomerular crescents, and vascular intimal thickening; other recognitions such as glomerular membranous deposits, vascular amyloidosis, and peritubular capillaritis will require confirmation in larger cohorts.

Conclusion

This study demonstrates the potential of D-FF-OCT imaging for on-site analysis of kidney biopsies, providing rapid and high-resolution images without extensive sample preparation.

Abstract Image

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动态全视野光学相干断层扫描肾活检快速现场组织病理学分析

肾脏组织学准备需要一个多步骤的过程，通常导致延迟的结果。本研究引入动态全场光学相干断层扫描（D-FF-OCT）作为一种无标记的替代方法，克服了传统组织病理学在现场肾脏病理评估中的局限性。方法纳入两个患者队列，共纳入31例患者；一组患者需要进行移植肾活检，另一组患者需要进行原生肾活检。前瞻性地收集临床和生物学资料。采用常规染色和动态D-FF-OCT成像对肾活检进行组织病理学分析。结果d - ff - oct能够识别大多数肾脏结构。结果显示，该技术与常规染色在评估间质纤维化（IF）方面具有显著相关性(r = 0.61, P <；0.001)和肾小管萎缩（TA） (r = 0.60, P <；0.001)。虽然可以发现许多病变，如间质炎症、急性小管坏死、肾小球新月形和血管内膜增厚；其他如肾小球膜性沉积、血管淀粉样变性和小管周围毛细血管炎需要在更大的队列中得到证实。本研究证明了D-FF-OCT成像在肾脏活检现场分析中的潜力，无需大量的样品制备，即可提供快速、高分辨率的图像。

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来源期刊

Kidney International Reports Medicine-Nephrology

CiteScore

7.70

自引率

3.30%

发文量

1578

审稿时长

8 weeks

期刊介绍： Kidney International Reports, an official journal of the International Society of Nephrology, is a peer-reviewed, open access journal devoted to the publication of leading research and developments related to kidney disease. With the primary aim of contributing to improved care of patients with kidney disease, the journal will publish original clinical and select translational articles and educational content related to the pathogenesis, evaluation and management of acute and chronic kidney disease, end stage renal disease (including transplantation), acid-base, fluid and electrolyte disturbances and hypertension. Of particular interest are submissions related to clinical trials, epidemiology, systematic reviews (including meta-analyses) and outcomes research. The journal will also provide a platform for wider dissemination of national and regional guidelines as well as consensus meeting reports.