Seroprevalence of Trichinella spp. in hunting dogs from southern Italy

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-05-05 DOI:10.1016/j.vprsr.2025.101276

Alessia Ricci , Marcos Antônio Bezerra-Santos , Alessandra Ludovisi , Irene Tartarelli , Gianluca Marucci , Adriano Casulli , Giovanni Sgroi , Vincenzo Veneziano , Riccardo Paolo Lia , Jairo Alfonso Mendoza-Roldan , Domenico Otranto

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Abstract

Wildlife plays a crucial role in maintaining the sylvatic cycle of Trichinella spp., representing an important source of infection for humans through the consumption of infected meat. Due to their scavenging behaviour and close interaction with wildlife, hunting dogs can be exposed to Trichinella spp. by ingesting muscle tissue containing infective larvae. In this study, we assessed the seroprevalence of Trichinella spp. in hunting dogs from southern Italy. Serum samples (n = 270) were collected from dogs across different areas of the Basilicata region and screened for anti-Trichinella IgG antibodies by an enzyme-linked immunosorbent assay (ELISA), followed by a confirmatory western blot (WB). Anti-Trichinella IgG antibodies were detected in 34.8 % (n = 94/270; 95 % CI: 0.29–0.41) by ELISA test, of which 59.6 % (n = 56/94; 95 % CI: 0.49–0.69) were confirmed by WB, resulting in an overall seroprevalence of 20.7 % (n = 56/270; 95 % CI: 0.16–0.26). Results show that hunting dogs may act as sentinels for monitoring the circulation of Trichinella spp., thereby assessing the potential risk for humans. Enhanced awareness among hunters and improved carcass handling practices are essential to mitigate the risk of transmission, reinforcing the need for an integrated control strategy approach.

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意大利南部猎犬中旋毛虫的血清流行率

野生动物在维持旋毛虫的森林循环中起着至关重要的作用，旋毛虫是人类通过食用受感染的肉类而感染的重要来源。由于它们的食腐行为和与野生动物的密切接触，猎犬可能通过摄入含有传染性幼虫的肌肉组织而暴露于旋毛虫。在这项研究中，我们评估了旋毛虫在意大利南部猎犬中的血清患病率。从巴西利卡塔地区不同地区的狗中采集血清样本（n = 270），通过酶联免疫吸附试验（ELISA）筛选抗旋毛虫IgG抗体，然后进行验证性免疫印迹（WB）。检出抗旋毛虫IgG抗体的占34.8% (n = 94/270；95% CI: 0.29-0.41)，其中59.6% (n = 56/94；95% CI: 0.49-0.69)经WB证实，总血清患病率为20.7% (n = 56/270；95% ci: 0.16-0.26)。结果表明，猎犬可以作为哨兵监测旋毛虫的传播，从而评估其对人类的潜在风险。提高猎人的认识和改进胴体处理方法对于减轻传播风险至关重要，从而加强了采取综合控制战略方法的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).