Novel Disk diffusion Assay for Filamentous Fungi Susceptibility to antifungals (DAFFS)

IF 1.7 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-05-06 DOI:10.1016/j.mimet.2025.107145

Salustra S. Urbin, Hailey Casey, Anna P.C. Price, Annette E. LaBauve, Carolyn L. Fisher

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Abstract

Filamentous fungi are recognized as significant human pathogens, particularly in immunocompromised populations. The rise in antifungal resistance and geographical expansion due to environmental changes, including climate change, further complicates treatment efforts. Existing antifungal susceptibility testing methods often face challenges when applied to filamentous fungi. Variability in results, extended incubation times, and difficulties in standardization can compromise broth dilution and disk diffusion assays. Here, we present an improved method, the Disk diffusion Assay for Filamentous Fungi Susceptibility to antifungals (DAFFS), designed to overcome current challenges in antifungal susceptibility testing for filamentous fungi. DAFFS integrates optimized elements from standard methods for more efficient screening of filamentous fungi for susceptibility to established and novel antifungals. This approach offers a streamlined, reliable alternative for assessing fungal drug susceptibility by addressing the limitations of current testing methods.

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新型盘片扩散法测定丝状真菌对抗真菌药敏

丝状真菌被认为是重要的人类病原体，特别是在免疫功能低下的人群中。由于包括气候变化在内的环境变化，抗真菌药物耐药性的上升和地理范围的扩大使治疗工作进一步复杂化。现有的抗真菌药敏试验方法在应用于丝状真菌时往往面临挑战。结果的可变性、培养时间的延长和标准化的困难会影响肉汤稀释和圆盘扩散测定。在这里，我们提出了一种改进的方法，丝状真菌抗真菌药敏试验（DAFFS），旨在克服目前丝状真菌抗真菌药敏试验的挑战。DAFFS整合了来自标准方法的优化元素，以更有效地筛选丝状真菌对已建立的和新型抗真菌药物的敏感性。这种方法通过解决当前测试方法的局限性，为评估真菌药物敏感性提供了一种简化、可靠的替代方法。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.