Transient expression of PRISEs and Trichoderma-mediated elicitation promote iridoid production in Nepeta sibirica L.

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-05-05 DOI:10.1016/j.plaphy.2025.109986

Neda Popović , Dragana Matekalo , Dejan Stojković , Marijana Skorić , Uroš Gašić , Jelena Božunović , Milica Milutinović , Luka Petrović , Jasmina Nestorović Živković , Slavica Dmitrović , Boban Anđelković , Milena Dimitrijević , Tijana Banjanac , Jovana Hrustić , Danijela Mišić

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Abstract

The genus Nepeta is the sole representative in the plant kingdom that produces nepetalactones, a group of iridoids with a unique stereochemistry, which play key roles in plant defense and ecological interactions. This study investigates N. sibirica L., a species rich in cis,trans-nepetalactone and 1,5,9-epi-deoxyloganic acid, aiming to enhance production of these bioactive iridoids by two alternative strategies: transient expression of key iridoid biosynthesis-related genes and fungal elicitation. In vitro treatments with Trichoderma harzianum and T. viride promoted iridoid production in N. sibirica leaves. It appears that regulatory proteins COI1, MYC2, and YABBY5 provoke coordinated upregulation of the early iridoid pathway genes (NsGPPS, NsGES, NsG8H, Ns8HGO), and of NsMLPL, thus stimulating metabolic flux through the iridoid pathway and providing substrates for the downstream steps mediated by NsISY, NsNEPS1, and NsNEPS2. The N. sibirica PRISE orthologue (NsPRISE) is closely related phylogenetically to the Family 1 isoforms known as P5βRs. However, its ISY-like activity was confirmed through in vitro assays with recombinant proteins expressed heterologously in E. coli. Transient overexpression experiments, which comparatively analysed in planta function of homologous NsPRISE and previously characterized ISY and PRISE orthologues from other Nepeta species, suggested possible in vivo residual ISY-like activity of NsPRISE and its involvement in iridoid production. The current study recognized N. sibirica as a plant susceptible to agroinfiltration, with iridoid metabolism that can be induced by pathogen attack, making it an ideal candidate for developing scalable systems for bioactive compounds production.

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瞬态表达和木霉介导的诱导可促进西伯利亚梨树环烯醚酮的产生。

Nepeta属是植物界唯一具有nepetalacones的代表植物，nepetalacones是一组具有独特立体化学的环烯醚萜类化合物，在植物防御和生态相互作用中起关键作用。本研究研究了一种富含顺式、反式内酯和1,5,9-外脱氧酸的植物N. sibirica L.，旨在通过两种替代策略：环烯醚酮生物合成相关关键基因的瞬时表达和真菌诱导来提高这些生物活性环烯醚酮的产量。哈兹木霉和绿霉在离体处理下促进了西伯利亚木叶片环烯醚萜的生成。由此可见，调控蛋白COI1、MYC2和YABBY5可引起环烯醚酮早期通路基因（NsGPPS、NsGES、NsG8H、Ns8HGO）和NsMLPL的协同上调，从而刺激环烯醚酮通路的代谢通量，并为NsISY、NsNEPS1和NsNEPS2介导的下游步骤提供底物。西伯利亚白鼬PRISE同源基因（NsPRISE）在系统发育上与家族1亚型P5βRs密切相关。然而，通过在大肠杆菌中异种表达的重组蛋白的体外实验，证实了其isy样活性。通过瞬时过表达实验，对比分析了NsPRISE同源基因在植物中的功能，并分析了其他荆芥属植物的ISY和PRISE同源基因，发现NsPRISE可能在体内具有ISY样活性，并参与环烯醚萜类化合物的产生。目前的研究认为西伯利亚野蓟是一种对农业渗透敏感的植物，具有可被病原体攻击诱导的环烯醚酮代谢，使其成为开发可扩展的生物活性化合物生产系统的理想候选者。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.