RNAi-mediated down-regulation of the endogenous GhAIP10.1 and GhAIP10.2 genes in transgenic cotton (Gossypium hirsutum) enhances the earliness and yield of flower buds

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-04-30 DOI:10.1016/j.plaphy.2025.109937

Marcos Fernando Basso , Thuanne Pires Ribeiro , Isabela Tristan Lourenço-Tessutti , Nelson Geraldo Oliveira , Reneida Aparecida Godinho Mendes , Niday Alline Nunes Fernandes , Fabricio Barbosa Monteiro Arraes , Carolina Vianna Morgante , Adriana Silva Hemerly , Maria Fatima Grossi-de-Sa

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引用次数: 0

Abstract

Armadillo BTB Arabidopsis protein 1 (AtABAP1) plays a central role in the cell cycle. ABAP1-interacting protein 10 (AtAIP10, a Snf1 kinase interactor-like protein) is a protein that interacts with AtABAP1. Down-regulation of the AtAIP10 gene in A. thaliana resulted in an altered cell cycle and increased photosynthesis, chlorophyll content, metabolites, plant growth, root system, seed yield, and drought tolerance. Herein, aimed to test whether the down-regulation of GhAIP10 genes can stimulate the cotton plants in a manner similar to those observed in A. thaliana. Cotton transgenic events containing transgenes carrying RNA interfering (RNAi) or artificial miRNA (amiRNA) strategies were successfully generated to down-regulate the endogenous GhAIP10.1 and GhAIP10.2 genes. From these 15 transgenic events, five RNAi-based transgenic lines and five amiRNA-based transgenic events were selected for further analyses. The down-regulation of the GhAIP10.1 and GhAIP10.2 genes was confirmed by real-time RT-PCR. Phenotypic and physiological analyses revealed that these transgenic lines exhibited earlier production and opening of flower buds, increased vegetative growth over time and root biomass, no reduction in susceptibility to root-knot nematodes, and improved drought tolerance indicated by a higher photosynthetic rate and better intrinsic water-use efficiency. Based on the high identity of amino acid sequences, motifs, domains, subcellular localization, tertiary structure, down-regulation of GhABAP1 (partner of GhAIP10), up-regulation of GhCdt1 (a marker of the ABAP1 network), up-regulation of GhCyclinB1 (a marker of the cell cycle), up-regulation of GhAP3 (involved in vegetative to reproductive transition), and the up-regulation of CAB3, NDA1, DJC22, and DNAJ11 genes (involved in plant resilience) suggested that GhAIP10.1 and GhAIP10.2 proteins may act in cotton similarly to the AtAIP10 protein in A. thaliana. Furthermore, GhAIP10.1 and GhAIP10.2 genes are suggested as biotechnological targets for cotton genetic engineering based on genome editing.

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rnai介导的转基因棉花（Gossypium hirsutum）内源GhAIP10.1和GhAIP10.2基因的下调提高了花蕾的早熟性和产量

犰狳BTB拟南芥蛋白1 （AtABAP1）在细胞周期中起核心作用。abap1相互作用蛋白10 （AtAIP10， Snf1激酶相互作用蛋白样蛋白）是一种与AtABAP1相互作用的蛋白。AtAIP10基因的下调导致拟蓝细胞周期改变，光合作用、叶绿素含量、代谢物、植物生长、根系、种子产量和耐旱性增加。本文旨在验证GhAIP10基因下调是否能以类似于拟南芥的方式刺激棉花植株。成功产生了含有携带RNA干扰（RNAi）或人工miRNA （amiRNA）策略的转基因棉花事件，下调了内源GhAIP10.1和GhAIP10.2基因。从这15个转基因事件中，选择5个基于rnai的转基因品系和5个基于amirna的转基因事件进行进一步分析。实时荧光定量pcr证实了GhAIP10.1和GhAIP10.2基因的下调。表型和生理分析表明，这些转基因品系的花蕾产生和开放时间提前，营养生长随时间增加，根系生物量增加，对根结线虫的敏感性不降低，光合速率和内在水分利用效率提高，耐旱性提高。基于氨基酸序列、基序、结构域、亚细胞定位、三级结构的高度一致性，下调GhABAP1 （GhAIP10的伴侣）、上调GhCdt1 （ABAP1网络的标记物）、上调GhCyclinB1（细胞周期的标记物）、上调GhAP3（参与营养向生殖过渡）以及上调CAB3、NDA1、DJC22，和DNAJ11基因（参与植物抗逆性）表明，GhAIP10.1和GhAIP10.2蛋白在棉花中的作用可能类似于AtAIP10蛋白在拟南芥中的作用。此外，GhAIP10.1和GhAIP10.2基因被建议作为棉花基因组编辑基因工程的生物技术靶点。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.