Functional characterization of 1-deoxy-D-xylulose-5-phosphate synthase (DXS) genes from Monarda citriodora establishes the key role of McDXS2 in specialized terpenoid biosynthesis

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-05-06 DOI:10.1016/j.plaphy.2025.109961

Priyanka Sharma , Mir Abdul Wajid , Koushik Pal , Mohd Fayaz , Aasim Majeed , Arvind Kumar Yadav , Deepika Singh , Sheetal Bhat , Wajid Waheed Bhat , Prashant Misra

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引用次数: 0

Abstract

Currently, limited information is available on the molecular basis of the biosynthesis of essential oil in the Monarda citriodora plant. Given the pivotal role of the MEP pathway in the biosynthesis of monoterpenes, in the present study, DXS genes have been functionally characterized from M. citriodora, for the first time. The CDS corresponding to four McDXS genes (1–4) were cloned, and their deduced proteins displayed distinct phylogenetic positioning. Using a bacterial complementation test, we demonstrated that all four McDXS genes encode functional DXS proteins. Based on the results obtained from phylogenetic analysis, tissue-specific expression analysis, and accumulation of monoterpenes, McDXS2 was identified as the candidate gene involved in the biosynthesis of monoterpenes of essential oil in M. citriodora. Transient overexpression and silencing of McDXS2 significantly modified the content of volatile monoterpenes in M. citriodora. Constitutive expression of McDXS2 in Nicotiana tabacum resulted in increased biosynthesis of specialized diterpenoids. Further, the exogenous treatment of MeJA, ABA, and GA₃ modulated the expression of McDXS2, and the content of the components of essential oil in M. citriodora. McDXS2 promoter activity was primarily restricted to the glandular trichomes of M. citriodora. The present work demonstrates that McDXS2 is primarily involved in the specialized terpenoid biosynthesis in M. citriodora.

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柠檬酸单胞菌1-脱氧-d -木醛糖-5-磷酸合成酶（DXS）基因的功能鉴定确定了McDXS2在特化萜类生物合成中的关键作用

目前，关于香茅植物精油生物合成的分子基础信息有限。鉴于MEP通路在单萜类生物合成中的关键作用，本研究首次从柠檬茅中对DXS基因进行了功能表征。克隆了4个McDXS基因（1 ~ 4）对应的CDS，其推导出的蛋白具有不同的系统发育定位。通过细菌互补测试，我们证明了所有四个McDXS基因都编码功能性DXS蛋白。基于系统发育分析、组织特异性表达分析和单萜类积累的结果，McDXS2被确定为柑橘精油单萜类生物合成的候选基因。McDXS2的瞬时过表达和沉默显著改变了香茅挥发性单萜的含量。McDXS2在烟草中的组成性表达导致特化二萜的生物合成增加。此外，外源处理MeJA、ABA和GA3可调节香茅中McDXS2的表达和精油成分的含量。McDXS2启动子活性主要局限于M. citriodora的腺毛。目前的研究表明McDXS2主要参与了M. citriodora特化萜类生物合成。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.