DsGGCT2-1 involved in the detoxification of Cd and Pb through the glutathione catabolism in Dianthus spiculifolius

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-04-30 DOI:10.1016/j.plaphy.2025.109976

Binbin Liu , Qi Wang , Jing Guan , Shufang Gong , Tuanyao Chai , Jingang Wang , Kun Qiao

{"title":"DsGGCT2-1 involved in the detoxification of Cd and Pb through the glutathione catabolism in Dianthus spiculifolius","authors":"Binbin Liu , Qi Wang , Jing Guan , Shufang Gong , Tuanyao Chai , Jingang Wang , Kun Qiao","doi":"10.1016/j.plaphy.2025.109976","DOIUrl":null,"url":null,"abstract":"<div><div>Toxic heavy metals seriously affect plant growth and human health. Among the heavy metals, cadmium (Cd) and lead (Pb) are serious pollutants. <em>Dianthus spiculifolius</em> has strong tolerance to, and an ability to accumulate, heavy metals. Therefore, it has potential applications as a heavy metal hyperaccumulator. Gamma glutamylcyclotransferase (GGCT) is a key enzyme in maintaining glutathione homeostasis, and it plays a role in plant growth and development and in responses to various stresses. Previously, <em>DsGGCT2-1</em> was identified as a gene showing significantly increased transcript levels in response to Cd and Pb by transcriptome analysis. In this study, DsGGCT2-1 was confirmed to increase the Cd and Pb tolerance of transgenic yeast, <em>Arabidopsis,</em> and <em>Dianthus</em>, decrease the their accumulation in <em>Dianthus</em>. Overexpression of <em>DsGGCT2-1</em> in <em>D. spiculifolius</em> plants resulted in increased GGCT activity, higher glutamate (Glu), and glutathione (GSH) content. The results suggest that more Glu is synthesized to maintain GSH homeostasis through the activity of GGCT2-1 in the glutamyl cycle, and the generated GSH is used to chelate with toxic heavy metals, and reduce the toxicity of heavy metals in the cytoplasm. These findings will be useful for devising strategies to remediate heavy metal-polluted soils, and for breeding plants that tolerate heavy metals.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"225 ","pages":"Article 109976"},"PeriodicalIF":6.1000,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942825005042","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Toxic heavy metals seriously affect plant growth and human health. Among the heavy metals, cadmium (Cd) and lead (Pb) are serious pollutants. Dianthus spiculifolius has strong tolerance to, and an ability to accumulate, heavy metals. Therefore, it has potential applications as a heavy metal hyperaccumulator. Gamma glutamylcyclotransferase (GGCT) is a key enzyme in maintaining glutathione homeostasis, and it plays a role in plant growth and development and in responses to various stresses. Previously, DsGGCT2-1 was identified as a gene showing significantly increased transcript levels in response to Cd and Pb by transcriptome analysis. In this study, DsGGCT2-1 was confirmed to increase the Cd and Pb tolerance of transgenic yeast, Arabidopsis, and Dianthus, decrease the their accumulation in Dianthus. Overexpression of DsGGCT2-1 in D. spiculifolius plants resulted in increased GGCT activity, higher glutamate (Glu), and glutathione (GSH) content. The results suggest that more Glu is synthesized to maintain GSH homeostasis through the activity of GGCT2-1 in the glutamyl cycle, and the generated GSH is used to chelate with toxic heavy metals, and reduce the toxicity of heavy metals in the cytoplasm. These findings will be useful for devising strategies to remediate heavy metal-polluted soils, and for breeding plants that tolerate heavy metals.

Abstract Image

查看原文本刊更多论文

DsGGCT2-1参与石竹谷胱甘肽分解代谢对Cd和Pb的解毒作用

有毒重金属严重影响植物生长和人体健康。在重金属中，镉（Cd）和铅（Pb）是严重的污染物。石竹对重金属有较强的耐受性和积累能力。因此，它作为重金属超蓄能器具有潜在的应用前景。谷氨酰环转移酶（Gamma glutamylcyclotransferase， GGCT）是维持谷胱甘肽稳态的关键酶，在植物生长发育和对各种胁迫的响应中发挥重要作用。此前，通过转录组分析，DsGGCT2-1被鉴定为一个对Cd和Pb的转录水平显著增加的基因。本研究证实，DsGGCT2-1能提高转基因酵母、拟南芥和石竹对Cd和Pb的耐受性，降低其在石竹中的积累。DsGGCT2-1在金丝桃中的过表达导致GGCT活性升高，谷氨酸（Glu）和谷胱甘肽（GSH）含量升高。结果表明，通过谷氨酰循环中GGCT2-1的活性，可以合成更多的Glu来维持谷胱甘肽的稳态，生成的谷胱甘肽用于与有毒重金属螯合，降低细胞质中重金属的毒性。这些发现将有助于制定修复重金属污染土壤的策略，以及培育耐受重金属的植物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.