Julia Pedreira-Rincón, Lourdes Rivas, Joan Comenge, Vasso Skouridou, Daniel Camprubí-Ferrer, Jose Muñoz, Ciara K. O'Sullivan, Alejandro Chamorro-Garcia and Claudio Parolo
{"title":"A comprehensive review of competitive lateral flow assays over the past decade†","authors":"Julia Pedreira-Rincón, Lourdes Rivas, Joan Comenge, Vasso Skouridou, Daniel Camprubí-Ferrer, Jose Muñoz, Ciara K. O'Sullivan, Alejandro Chamorro-Garcia and Claudio Parolo","doi":"10.1039/D4LC01075B","DOIUrl":null,"url":null,"abstract":"<p >Competitive lateral flow assays (LFAs) provide a versatile and cost-effective platform for detecting a wide range of molecular targets across fields such as healthcare, food safety, and environmental monitoring, particularly for small analytes or single epitopes that lack suitable bioreceptor pairs. However, the interpretation of competitive LFAs can be challenging due to their counterintuitive output, where the absence of a test line signifies the presence of the target. In this review, we present a comprehensive overview of the fundamental strategies underlying competitive LFAs, explore the mathematical models that quantify assay performance, and outline the critical parameters involved in their design and optimization. We further highlight notable applications and discuss methods to enhance the user experience through improved result interpretation and user-centric design. By consolidating current knowledge and best practices, this work will serve as a valuable reference for researchers and developers seeking to refine the usability, reliability, and effectiveness of competitive LFAs.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 11","pages":" 2578-2608"},"PeriodicalIF":5.4000,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d4lc01075b?page=search","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lab on a Chip","FirstCategoryId":"5","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/lc/d4lc01075b","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Competitive lateral flow assays (LFAs) provide a versatile and cost-effective platform for detecting a wide range of molecular targets across fields such as healthcare, food safety, and environmental monitoring, particularly for small analytes or single epitopes that lack suitable bioreceptor pairs. However, the interpretation of competitive LFAs can be challenging due to their counterintuitive output, where the absence of a test line signifies the presence of the target. In this review, we present a comprehensive overview of the fundamental strategies underlying competitive LFAs, explore the mathematical models that quantify assay performance, and outline the critical parameters involved in their design and optimization. We further highlight notable applications and discuss methods to enhance the user experience through improved result interpretation and user-centric design. By consolidating current knowledge and best practices, this work will serve as a valuable reference for researchers and developers seeking to refine the usability, reliability, and effectiveness of competitive LFAs.
期刊介绍:
Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.