Fusarium cross-infection in medicinal herbs alters rhizosphere microbiomes and disrupts mycorrhizal functions under soil physicochemical imbalances

IF 3.9 2区农林科学 Q1 AGRONOMY

Plant and Soil Pub Date : 2025-05-08 DOI:10.1007/s11104-025-07504-5

Andéole Niyongabo Turatsinze, Xiaofan Xie, Ailing Ye, Gaofeng Chen, Yun Wang, Liang Yue, Qin Zhou, Lingling Wu, Meilan Zhang, Zongyu Zhang, Jiecai Zhao, Yuexia Sha, Yubao Zhang, Ruoyu Wang

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Abstract

Background and aims

Fusarium root rot and wilt affect medicinal herbs in Gansu Province, China, despite extended crop rotations. This study investigated the cross-pathogenicity of Fusarium species isolated from Angelica sinensis (Danggui), Codonopsis pilosula (Dangshen), and Astragalus mongholicus (Huangqi).

Methods

Of 83 fungal isolates recovered, 69.8% were identified as Fusarium spp., through ITS, TEF1-α, and RPB2 sequencing, clustering into Fusarium oxysporum (FOSC, 36.2%), Fusarium solani (FSSC, 31%), and Fusarium tricinctum (FTSC, 22.4%) species complexes. Representative strains (F. oxysporum DSH27, F. solani HQ123, F. tricinctum DG105) were tested for cross-pathogenicity in greenhouse and field trials. Rhizosphere microbial dynamics, including fungal and bacterial community diversity, functional guilds, and soil physicochemical properties, were analyzed.

Results

Fusarium strains exhibited varying aggressiveness, highest on original hosts, while cross-infective hosts showed less to moderate severity. Infections disrupted rhizosphere networks, increasing pathotrophic dominance over arbuscular mycorrhizal functions. Sequencing showed reduced fungal and bacterial operational taxonomic units (OTUs), with distinct clustering of infected vs. non-infected rhizospheres. Pathogenic fungal genera Fusarium positively correlated with disease incidence, while beneficial fungal genera Mortierella and bacterial genera RB41 showed negative correlations. Infected soils exhibited significant changes in total carbon, available phosphorus, manganese, and zinc, correlating with microbial dynamics and disease severity.

Conclusion

This study links Fusarium cross-infection with rhizosphere microbial network disruptions, including the loss of arbuscular mycorrhizal fungi (AMF) functions under altered soil physicochemical conditions in medicinal herbs. These findings uncover the systematic cross-pathogenicity of Fusarium species, highlighting the need for AMF-based strategies and integrated soil management to mitigate its impact.

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镰刀菌交叉侵染改变了根际微生物群，破坏了土壤理化失衡条件下菌根的功能

背景与目的镰刀菌根腐病和枯萎病影响中国甘肃省的草药，尽管延长了作物轮作。研究了当归、党参和黄芪中镰刀菌的交叉致病性。方法83株真菌中，经ITS、TEF1-α和RPB2测序，鉴定为镰刀菌69.8%，聚类为尖孢镰刀菌（Fusarium oxysporum, 36.2%）、番茄镰刀菌（Fusarium solani, 31%）和tricinctum镰刀菌（Fusarium solani, 22.4%）。在温室和田间试验中，对代表性菌株尖孢菌DSH27、茄枯菌HQ123、tricinctum DG105进行了交叉致病性检测。分析了根际微生物动态，包括真菌和细菌群落多样性、功能行会和土壤理化性质。结果镰刀菌具有不同程度的侵袭性，对原始宿主的侵袭性最强，而交叉感染宿主的侵袭性较弱至中等。感染破坏了根际网络，增加了对丛枝菌根功能的致病性优势。测序显示真菌和细菌的操作分类单位（OTUs）减少，感染根际与未感染根际具有明显的聚类。致病性真菌镰刀菌属与发病率呈正相关，有益真菌摩梯菌属和细菌属RB41呈负相关。感染土壤的总碳、速效磷、锰和锌表现出显著的变化，与微生物动态和疾病严重程度相关。结论在改变土壤理化条件下，镰刀菌交叉感染与中药材根际微生物网络破坏有关，包括丛枝菌根真菌（AMF）功能的丧失。这些发现揭示了镰刀菌物种的系统性交叉致病性，强调了基于amf的策略和综合土壤管理的必要性，以减轻其影响。

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来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.