Strontium-Doped Implant Osseointegration Under Dry Preservation Conditions: In Vitro and Rat Model-Based In Vivo Studies

IF 3.7 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical Implant Dentistry and Related Research Pub Date : 2025-05-08 DOI:10.1111/cid.70051

Jimin Jiang, Yingyu Zeng, Ibrahim El Khalil Bouhamed, Fangyuan Cai, Tianning Ye, Hui Wang, Sang Hyun Lee, Qianming Chen, Fuming He

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引用次数: 0

Abstract

Objective

This study aims to develop an innovative dry preservation surface modification for strontium-modified pure titanium implants to prevent surface degradation by hydrocarbon pollutants in the air and to enhance osseointegration.

Methods

An optimal parameter dry preservation surface modification was established by immersing samples in a 0.1 M KCl + 0.1 M SrCl₂ mixed solution for 12 h, followed by air-drying and storing in sealed glass vials, as the strontium-doped dry preservation (SrP) group. Surface characteristics were analyzed using appropriate instrumentation. Cell adhesion, proliferation, and osteogenic differentiation were assessed in in vitro studies. Forty-eight male Sprague–Dawley rats were included in in vivo studies, with a total of 96 implants placed bilaterally in the tibiae. Micro-CT, removal torque tests, and histological analyses after 4 or 8 weeks were conducted in order to evaluate the osseointegration in each group.

Results

Four distinct groups were involved in this study: the SrP group, the freshly prepared strontium-doped (SrF) group, the strontium-doped group with no dry preservation (SrN), and the modified sand-blasted large grit acid-etched (modSLA) group. After 6 months of storage, the SrP surface maintained favorable hydrophilicity, micro-nano morphology, and consistent strontium ion release similar to SrF. Moreover, it maintained similar levels of cell proliferation and osteogenic differentiation as SrF while exhibiting significantly better performance than SrN in in vitro studies (p < 0.05). Additionally, after 12 months of storage, it demonstrated excellent osseointegration comparable to the SrF group while significantly outperforming SrN in in vivo studies 4 weeks post-surgery (p < 0.05).

Conclusion

The dry preservation surface modification successfully maintained the nanotopography and hydrophilicity of strontium-doped pure titanium implants during 6–12 months of storage, and effectively mitigated the premature release of strontium ions associated with wet preservation methods, thereby significantly preserving the osseointegration performance of strontium-doped titanium implants.

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干燥保存条件下掺锶种植体骨整合：体外和基于大鼠模型的体内研究

目的研究一种新型的干保存纯钛钛种植体表面改性方法，以防止空气中碳氢化合物污染物对其表面的降解，增强其骨整合能力。方法将样品在0.1 M KCl + 0.1 M SrCl2混合溶液中浸泡12 h，然后风干，密封玻璃瓶中保存，作为掺锶干保存（SrP）组，建立最佳参数干保存表面修饰。使用适当的仪器分析表面特征。在体外研究中评估了细胞粘附、增殖和成骨分化。48只雄性Sprague-Dawley大鼠被纳入体内研究，共96个植入物放置在双侧胫骨。4周或8周后进行Micro-CT、去除扭矩测试和组织学分析，以评估各组骨整合情况。结果本研究分为四个不同的组：SrP组、新鲜制备的掺锶（SrF）组、无干燥保存的掺锶组（SrN）和改性喷砂大粒度酸蚀（modSLA）组。经过6个月的储存，SrP表面保持了与SrF相似的良好亲水性、微纳形貌和一致的锶离子释放。此外，在体外研究中，它保持了与SrF相似的细胞增殖和成骨分化水平，且表现出明显优于SrN的性能（p < 0.05）。此外，在12个月的储存后，它表现出与SrF组相当的良好骨整合，而在手术后4周的体内研究中，它的表现明显优于SrN （p < 0.05）。结论干法保存表面修饰在6 ~ 12个月的保存时间内，成功维持了掺锶纯钛种植体的纳米形貌和亲水性，有效缓解了湿法保存相关的锶离子过早释放，从而显著保持了掺锶纯钛种植体的骨整合性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Implant Dentistry and Related Research 医学-牙科与口腔外科

CiteScore

6.00

自引率

13.90%

发文量

103

审稿时长

4-8 weeks

期刊介绍： The goal of Clinical Implant Dentistry and Related Research is to advance the scientific and technical aspects relating to dental implants and related scientific subjects. Dissemination of new and evolving information related to dental implants and the related science is the primary goal of our journal. The range of topics covered by the journals will include but be not limited to: New scientific developments relating to bone Implant surfaces and their relationship to the surrounding tissues Computer aided implant designs Computer aided prosthetic designs Immediate implant loading Immediate implant placement Materials relating to bone induction and conduction New surgical methods relating to implant placement New materials and methods relating to implant restorations Methods for determining implant stability A primary focus of the journal is publication of evidenced based articles evaluating to new dental implants, techniques and multicenter studies evaluating these treatments. In addition basic science research relating to wound healing and osseointegration will be an important focus for the journal.