Characterization of Fecal Microbial Communities in Patients With Type 2 Diabetes Mellitus Combined With Helicobacter pylori Infection

IF 4.3 2区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Helicobacter Pub Date : 2025-05-07 DOI:10.1111/hel.70041

Xiaoyan He, Han Chen, Fengdan Chen, Wei Su, Yan Wang, Die Hu, Jianwen Hu, Xiaoying Zhou

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Abstract

Background

Helicobacter pylori (H. pylori) infection has the capacity to alter the gut microbiota composition. There is a significant correlation between H. pylori infection and type 2 diabetes mellitus (T2DM). Further research is necessary to explore whether gut microbiota plays a role in the relationship between H. pylori and T2DM.

Method

Fecal samples were obtained from 44 patients with T2DM, including 20 who tested positive for H. pylori and 24 who tested negative. Intestinal microbiota composition was analyzed via 16S rRNA V3-V4 amplicon sequencing. Differences in microbial distribution and significant microbial biomarkers were identified between H. pylori positive and negative groups. A Spearman correlation analysis assessed the relationship between intestinal microbiota and glycemic parameters. Additionally, PICRUSt2 was used to predict intestinal bacterial functions.

Results

Results indicate that in H. pylori (+) T2DM patients, HbA1c levels were significantly higher (8.9% vs. 8.1%, p = 0.021), while both the C-peptide peak (3.70 vs. 5.98 ng/mL, p = 0.040) and fasting C-peptide levels (1.42 vs. 2.31 ng/mL, p = 0.008) were significantly lower compared to H. pylori (−) T2DM groups. A total of 11 colonic phyla and 100 genera were identified in all fecal samples. In groups positive for H. pylori, there was a significant enrichment of the phylum Proteobacteria, while the genera Lactobacillus, Butyricimonas, and Akkermansia were significantly reduced (all p < 0.05). Correlation analysis showed that the abundance of the genera Butyricimonas (p = 0.01) and Akkermansia (p = 0.048) were negatively correlated with fasting plasma glucose. KEGG pathway analysis indicated a significant enrichment of methylmalonyl-CoA mutase and succinyl-CoA in H. pylori-infected T2DM patients.

Conclusions

This study suggests that T2DM patients with H. pylori infection exhibit more impaired pancreatic islet function potentially due to H. pylori-induced alterations in the gut microbiota.

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2型糖尿病合并幽门螺杆菌感染患者粪便微生物群落特征

背景幽门螺杆菌（h.p ylori）感染能够改变肠道菌群组成。幽门螺杆菌感染与2型糖尿病（T2DM）有显著相关性。肠道菌群是否在幽门螺杆菌与T2DM的关系中发挥作用，还需要进一步的研究。方法对44例T2DM患者进行粪便标本采集，其中幽门螺旋杆菌阳性20例，阴性24例。通过16S rRNA V3-V4扩增子测序分析肠道菌群组成。在幽门螺杆菌阳性组和阴性组之间，微生物分布和显著的微生物生物标志物存在差异。Spearman相关分析评估了肠道微生物群与血糖参数之间的关系。此外，PICRUSt2被用于预测肠道细菌功能。结果与幽门螺杆菌（+）型T2DM组相比，HbA1c水平显著升高（8.9% vs. 8.1%, p = 0.021），而c肽峰值（3.70 vs. 5.98 ng/mL, p = 0.040）和空腹c肽水平（1.42 vs. 2.31 ng/mL, p = 0.008）均显著降低。所有粪便标本共鉴定出11个结肠门100个属。在幽门螺杆菌阳性的组中，变形杆菌门的数量显著增加，而乳酸杆菌、丁酸单胞菌和Akkermansia属的数量显著减少（p < 0.05）。相关分析显示，丁酸单胞菌属丰度（p = 0.01）和Akkermansia属丰度（p = 0.048）与空腹血糖呈负相关。KEGG通路分析显示，在幽门螺杆菌感染的T2DM患者中，甲基丙二酰辅酶a变化酶和琥珀酰辅酶a显著富集。结论：本研究表明，幽门螺杆菌感染的T2DM患者胰岛功能受损更严重，可能是由于幽门螺杆菌诱导的肠道微生物群改变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Helicobacter 医学-微生物学

CiteScore

8.40

自引率

9.10%

发文量

审稿时长

2 months

期刊介绍： Helicobacter is edited by Professor David Y Graham. The editorial and peer review process is an independent process. Whenever there is a conflict of interest, the editor and editorial board will declare their interests and affiliations. Helicobacter recognises the critical role that has been established for Helicobacter pylori in peptic ulcer, gastric adenocarcinoma, and primary gastric lymphoma. As new helicobacter species are now regularly being discovered, Helicobacter covers the entire range of helicobacter research, increasing communication among the fields of gastroenterology; microbiology; vaccine development; laboratory animal science.