A novel strategy for ultrasensitive detection and effective inactivation of Staphylococcus aureus based on Fe3O4-QCS-PEI-Cu-aptamer and ladder-branch HCR

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-05-07 DOI:10.1007/s00604-025-07184-z

Dongxia Ren, Lin Wang, Hua Wei, Shijie Mu, Longfei Yang

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引用次数: 0

Abstract

Bacterial infection is one of the leading causes of human death globally and is considered to be an urgent public health challenge. It is important to accurately and rapidly detect and kill pathogenic microorganisms. Therefore, a multifunctional platform for sensitive detection and efficient elimination of pathogenic bacteria should be developed to reduce the threat of bacteria. In this study, an integrated strategy based on Fe₃O₄-quaternary ammonium chitosan-polyetherimide-Cu-aptamer (Fe₃O₄-QCS-PEI-Cu-aptamer) and ladder-branch hybridization chain reaction (HCR) was proposed for the detection and inactivation of Staphylococcus aureus (S. aureus). The aptamer-complementary chain (aptamer-CP) conjugated to the surface of Fe₃O₄-QCS-PEI-Cu induces CP release in the presence of S. aureus, further initiating the ladder-branch HCR reaction and achieving signal amplification. Under optimal conditions, the developed sensor demonstrated a limit of detection (LOD) of 3 CFU/mL for S. aureus and exhibited high selectivity against several other pathogenic bacteria. Moreover, the Fe₃O₄-QCS-PEI-Cu-aptasensor showed potent antibacterial properties under near infrared (NIR) irradiation, achieving an antimicrobial efficiency of 99.946%. This strategy synergistically combined the high-sensitivity molecular detection with the antibacterial activity of novel nanomaterials activated by near infrared light, providing a new insight into the prevention of bacterial infections.

Graphical Abstract

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基于fe3o4 - qcs - pei - cu -适体和阶梯分支HCR的金黄色葡萄球菌超灵敏检测和有效灭活新策略

细菌感染是全球人类死亡的主要原因之一，被认为是一项紧迫的公共卫生挑战。准确、快速地检测和杀灭病原微生物具有重要意义。因此，为了减少细菌的威胁，需要开发一种灵敏检测和高效消灭病原菌的多功能平台。本研究提出了一种基于fe3o4 -季铵盐壳聚糖-聚醚酰亚胺- cu -适体（fe3o4 - qcs - pei - cu -适体）和阶梯支杂交链反应（HCR）的检测和灭活金黄色葡萄球菌（S. aureus）的综合策略。在金黄色葡萄球菌存在的情况下，与Fe3O4-QCS-PEI-Cu表面结合的适体互补链（aptamer-CP）诱导CP释放，进一步启动阶梯-分支HCR反应，实现信号放大。在最佳条件下，该传感器对金黄色葡萄球菌的检出限（LOD）为3 CFU/mL，对其他几种致病菌具有较高的选择性。此外，fe3o4 - qcs - pei - cu - aptassensor在近红外（NIR）照射下表现出较强的抗菌性能，抗菌效率达到99.946%。该策略将高灵敏度分子检测与近红外光激活的新型纳米材料的抗菌活性协同结合，为预防细菌感染提供了新的视角。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.