GIS mediates GA signaling to directly target the expression of SPL15 to regulate trichome development in Arabidopsis thaliana

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-04-30 DOI:10.1016/j.plaphy.2025.109975

Chunyan Yang , Lili Sun , Muhammad Umair Yasin , Haider Zulqarnain , Bahar Ali , Yihua Liu , Bohan Liu , Yinbo Gan

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引用次数: 0

Abstract

Trichome development in Arabidopsis thaliana is regulated by a complex network of genetic and hormonal signaling pathways. GA/DELLA/GISs and miR156/SPLs are the two main regulatory modules regulating trichome initiation in A. thaliana. However, it is not clear whether there is an interaction between them. Furthermore, GIS was the first gene in the GIS family to be identified as regulating trichome development through GA signal in A. thaliana. Nevertheless, how GIS regulates trichome development through GA signal is still unknown. In this study, we first illuminated that GIS could directly target the expression of SPL15 to regulate trichome development through GA signal. Our results showed that the loss of SPL15 function significantly increases trichome density, whereas its overexpression suppresses trichome formation. Moreover, exogenous GA treatment promotes trichome development in the SPL15 overexpressing lines. More importantly, molecular and genetic studies revealed that GIS acts upstream of SPL15 by binding to its promoter to regulate the trichome development in A. thaliana. These indicate that C2H2 transcriptional factor GIS may be the key bridge gene connecting miR156/SPLs and GA/DELLA/GIS modules to regulate trichome development in A. thaliana. These results reveal a novel pathway, GA-GIS-SPL15, to regulate trichome development through the GA pathway and extend our knowledge of the GA-GIS-SPL15 regulating network.

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GIS介导GA信号直接靶向SPL15的表达，调控拟南芥的毛状体发育

拟南芥的毛状体发育是由一个复杂的遗传和激素信号通路网络调控的。GA/DELLA/GISs和miR156/SPLs是拟蓝藻毛状体形成的两个主要调控模块。然而，目前尚不清楚它们之间是否存在相互作用。此外，GIS是GIS家族中第一个被发现通过GA信号调控拟南芥毛状体发育的基因。然而，GIS如何通过遗传信号调控毛状体发育仍是未知的。在本研究中，我们首次阐明了GIS可以直接靶向SPL15的表达，通过GA信号调控毛状体的发育。我们的研究结果表明，SPL15功能的缺失显著增加了毛状体密度，而其过表达则抑制了毛状体的形成。此外，外源GA处理促进SPL15过表达系的毛状体发育。更重要的是，分子和遗传学研究表明，GIS通过与SPL15的启动子结合，作用于SPL15的上游，调控拟南芥的毛状体发育。这表明C2H2转录因子GIS可能是连接miR156/SPLs和GA/DELLA/GIS模块调控拟南芥毛状体发育的关键桥接基因。这些结果揭示了一个新的通路GA- gis - spl15通过GA通路调控毛状体发育，并扩展了我们对GA- gis - spl15调控网络的认识。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.