Cloning, expression, and functional characterization of three f 6-hydroxyalizarin glycosyltransferases from Rheum palmatum

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-05-05 DOI:10.1016/j.plaphy.2025.109987

Jing Zou , Xin-Yu Zhao , Li-Hong Ji , Jian-Zhen Zou , Yuan-Yuan Han , Yi-Min Li , Meng-Meng Liu

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引用次数: 0

Abstract

Glycosyltransferases are key enzymes responsible for the glycosylation of natural products in plants. The roots and rhizomes of Rheum palmatum Diels, commonly known as rhubarb, are well-established in traditional Chinese medicine and are rich in diverse glycoside natural products. Despite this, no glycosyltransferase from R. palmatum had been molecularly and biochemically characterized until now. In this study, we report the identification and characterization of three novel glycosyltransferases (GTs) - RpUGT1, RpUGT6, and RpUGT30 - that mediate f 6-hydroxyalizarin glycoside biosynthesis. These enzymes exhibit regioselective glycosylation of β-OH anthraquinones and display substrate promiscuity, acting on at least six compounds to form O-glycosides. Molecular modeling and site-directed mutagenesis have identified critical residues essential for substrate binding and glycosylation. These results elucidate the pivotal enzymes and molecular mechanisms underlying 6-hydroxyalizarin biosynthesis in Polygonaceae plants, paving the way for the complete elucidation of this biosynthetic pathway and the construction of an artificial pathway for 6-hydroxyalizarin glycoside production.

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掌大黄3个f6 -羟基茜素糖基转移酶的克隆、表达及功能表征

糖基转移酶是植物天然产物糖基化的关键酶。大黄（Rheum palmatum Diels）的根和根茎，俗称大黄，在传统中医中有着悠久的历史，富含多种苷类天然产物。尽管如此，到目前为止，还没有对棕榈叶的糖基转移酶进行分子和生物化学表征。在这项研究中，我们报道了三种新的糖基转移酶（GTs）的鉴定和表征- RpUGT1， RpUGT6和RpUGT30 -介导6-羟基化苷苷的生物合成。这些酶表现出β-OH蒽醌的区域选择性糖基化和底物混杂性，作用于至少六种化合物形成o -糖苷。分子模型和定点诱变已经确定了底物结合和糖基化所必需的关键残基。这些结果阐明了蓼科植物6-羟基茜素生物合成的关键酶及其分子机制，为全面阐明该生物合成途径和构建6-羟基茜素糖苷人工合成途径奠定了基础。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.