Complexation with chicken yolk phosvitin modulates antibacterial activity of chitosan

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers Pub Date : 2025-05-02 DOI:10.1016/j.carbpol.2025.123691

Boris B. Berezin , Sergey N. Kulikov , Irina L. Zhuravleva , Marina G. Ezernitskaya , Vladimir E. Piskarev , Yurij A. Antonov , Evgeniya A. Bezrodnykh , Vladimir E. Tikhonov

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Abstract

In this study, it is shown for the first time that complexation with phosvitin does not restrict bacteriostatic activity of chitosan. Moreover, chitosan possesses the activity in the presence of phosvitin in acidic and alkalescent media. Besides, minimal inhibition concentrations of chitosan decrease in both acidic (pH 5.8) and alkalescent (pH 7.4) media, and chitosan shows two/fourfold higher bacteriostatic activity at 0.25–1.0 mg/ml phosvitin concentration in solution compared with that of chitosan alone due to an additive effect of chitosan and phosvitin, i.e. phosvitin modulates antimicrobial activity of chitosan in the acidic and alkalescent media. It is shown that the electrostatic and non-electrostatic forces are responsible for formation of soluble and insoluble chitosan/phosvitin complexes. Solely negatively charged complexes are formed in alkalescent medium. A faster interaction occurs in acidic medium, and insoluble complexes have five times as much phosvitin as the soluble ones. In their turn, soluble complexes enriched with chitosan are formed in both acidic and alkalescent media. It is presumed that a transfer of labile protons from phosvitin onto chitosan strengthens the electrostatic complexation between chitosan and phosvitin molecules. The finding can be promising for construction of phosvitin/chitosan-based drug-delivery systems and cosmetic compositions possessing antimicrobial activity.

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蛋黄磷维素与壳聚糖络合可调节壳聚糖的抑菌活性

本研究首次证明壳聚糖与磷维素的络合作用不限制壳聚糖的抑菌活性。此外，壳聚糖在酸性和碱性介质中都具有光维素存在时的活性。此外，壳聚糖在酸性（pH 5.8）和碱性（pH 7.4）培养基中的最小抑菌浓度均降低，且壳聚糖在溶液中磷维素浓度为0.25 ~ 1.0 mg/ml时的抑菌活性比单独使用壳聚糖高2 ~ 4倍，这是由于壳聚糖和磷维素的加性作用，即磷维素调节壳聚糖在酸性和碱性培养基中的抑菌活性。结果表明，静电和非静电力是形成可溶性和不可溶性壳聚糖/磷维素配合物的主要原因。带负电荷的配合物在碱性介质中形成。在酸性介质中发生更快的相互作用，不溶性络合物的光维素是可溶络合物的五倍。反过来，在酸性和碱性介质中都可以形成富含壳聚糖的可溶性配合物。据推测，不稳定的质子从磷维素转移到壳聚糖上，加强了壳聚糖和磷维素分子之间的静电络合作用。这一发现为构建磷维素/壳聚糖为基础的药物传递系统和具有抗菌活性的化妆品组合物提供了前景。

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.