Dietary calcium promotes growth, lipid metabolism and energy metabolism by activating the Ca2+-Camkkβ-AMPK signaling pathway in Pacific white shrimp (Litopenaeus vannamei)

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-05-03 DOI:10.1016/j.aquaculture.2025.742671

Yu He , Tiantian Xu , Min Jin , Xiaoru Chen , Yinzhao Zhang , Feng Tang , Lu Zhang , Tingting Zhu , Yi Wu , Jinlin Wang , Shuqin Li , Qicun Zhou

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引用次数: 0

Abstract

This study aimed to evaluate the effects of different dietary calcium levels on the growth, lipid and energy metabolism, and calcium-mediated Ca²⁺-Camkkβ-AMPK signaling pathway of Litopenaeus vannamei. Six isonitrogenous and isolipidic diets were formulated to contain different calcium levels, the analyzed calcium were 2.12 %, 2.27 %, 2.35 %, 2.47 %, 2.54 %, and 2.66 %, respectively. The results showed that shrimp fed diets with 2.35 % to 2.54 % calcium were significantly higher final weight (FW), percent weight gain (PWG), specific growth rate (SGR) and feed efficiency (FE) than those fed with the control diet (P < 0.05). The highest triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) in the hemolymph, the activities of the rate-limiting enzymes of succinate dehydrogenase (SDH), CA, citric acid (CA), creatine kinase (CK), pyruvate kinase (PK) and energy metabolism-related indicators (ATP, NADH, NAD⁺) in the hepatopancreas mitochondria were observed at shrimp fed diet with 2.47 % calcium (P < 0.05). The expression of genes related to the calcium signaling pathway (pka, cam, pde), molting (mih, ecr, flz-f1, ethr, chs, e75, ef-1α, chi2), energy metabolism (ampkα, ampkβ, ampkγ, acc1, mcd), tricarboxylic acid cycle (sdh, cs, aco, idh, mdh, fh), mitochondrial electron respiratory chain (ndh, cco, cox, atph), and lipid metabolism (fas, srebp) related genes were significantly upregulated at shrimp fed diet with 2.47 % calcium (P < 0.05). In conclusion, dietary calcium level of 2.47 % can promote molting and growth by activating the Ca²⁺-Camkkβ-AMPK signaling pathway, promoting the tricarboxylic acid cycle and energy metabolism, and promoting lipid metabolism of L. vannamei.

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饲料钙通过激活Ca2+-Camkkβ-AMPK信号通路促进凡纳滨对虾生长、脂质代谢和能量代谢

本研究旨在探讨不同饲料钙水平对凡纳滨对虾生长、脂质和能量代谢以及钙介导的Ca2+-Camkkβ-AMPK信号通路的影响。配制了6种不同钙含量的等氮等脂饲料，测定的钙含量分别为2.12%、2.27%、2.35%、2.47%、2.54%和2.66%。结果表明，钙含量为2.35% ~ 2.54%的对虾最终重（FW）、增重率（PWG）、特定生长率（SGR）和饲料效率（FE）显著高于对照组(P <；0.05)。血淋巴中甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）含量最高，肝胰腺线粒体中丁二酸脱氢酶（SDH）、CA、柠檬酸（CA）、肌酸激酶（CK）、丙酮酸激酶（PK）等限速酶活性和能量代谢相关指标（ATP、NADH、NAD+）活性均在2.47%钙(P <；0.05)。钙含量为2.47%时，钙信号通路（pka、cam、pde）、脱毛（mih、ecr、flz-f1、ethr、chs、e75、ef-1α、chi2）、能量代谢（ampkα、ampkβ、ampkγ、acc1、mcd）、三羧酸循环（sdh、cs、aco、idh、mdh、fh）、线粒体电子呼吸链（ndh、cco、cox、atph）和脂质代谢（fas、srebp）相关基因的表达显著上调(P <；0.05)。综上所述，饲粮钙水平2.47%可通过激活Ca2+-Camkkβ-AMPK信号通路、促进三羧酸循环和能量代谢、促进脂质代谢等途径促进凡纳梅脱毛和生长。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.