Multiscale characterization of jawbone treated with osteoporosis therapeutic agents

IF 3.3 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials Pub Date : 2025-04-30 DOI:10.1016/j.jmbbm.2025.107036

Keiichiro Watanabe , Cheol-Min Han , Allison R. Altman-Singles , Jie Liu , Xiaohan Guo , Ai Ni , Mason Bahador , Tala Ebrahimian , Jayoung Kim , Beth S. Lee , X. Sherry Liu , Do-Gyoon Kim

{"title":"Multiscale characterization of jawbone treated with osteoporosis therapeutic agents","authors":"Keiichiro Watanabe , Cheol-Min Han , Allison R. Altman-Singles , Jie Liu , Xiaohan Guo , Ai Ni , Mason Bahador , Tala Ebrahimian , Jayoung Kim , Beth S. Lee , X. Sherry Liu , Do-Gyoon Kim","doi":"10.1016/j.jmbbm.2025.107036","DOIUrl":null,"url":null,"abstract":"<div><div>The objective of the current study was to determine whether treatments of bisphosphonate (alendronate (ALN)), parathyroid hormone (PTH), and their combination have an effect on the jawbone in estrogen deficient rats. Six female rats (4-month-old) were used for each sham surgery (SHAM). Twenty-four rats (4-month-old) were ovariectomized and randomly assigned to four equal groups: saline injection (VEH), PTH following saline injection (VEH/PTH), bisphosphonate (ALN), or a combination (ALN/PTH). A hemimandible was randomly dissected from each rat for multiscale (10<sup>−2</sup> to 10<sup>−7</sup> m) characterization including static and dynamic mechanical stability of teeth in the alveolar socket, tissue mineral density distribution (TMD), and nanoindentation properties of the jawbone matrix. Most jawbone characteristics in OVX and its treatment groups were not significantly different from those of the SHAM group. The surface of alveolar bone (AB) surrounding teeth showed a trend of more erosion and addition of new bone tissues in the OVX rat groups compared to the SHAM group. All TMD parameters rapidly increased up to 60 μm from the periodontal ligament surrounding teeth regardless of the treatment groups. Treatments using each therapeutic agent and its combination did not substantially change those characteristics of jawbones in OVX rats. These findings are different from those of lumbar vertebrae in the same rats that showed a significant bone alteration by OVX and treatments. Thus, the current multiscale characterization of jawbone provides comprehensive information that can help better understand jawbone-specific responses to bone-related complications, including postmenopausal osteoporosis and bisphosphonate-related osteonecrosis of the jaw.</div></div>","PeriodicalId":380,"journal":{"name":"Journal of the Mechanical Behavior of Biomedical Materials","volume":"169 ","pages":"Article 107036"},"PeriodicalIF":3.3000,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Mechanical Behavior of Biomedical Materials","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1751616125001523","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}

引用次数: 0

Abstract

The objective of the current study was to determine whether treatments of bisphosphonate (alendronate (ALN)), parathyroid hormone (PTH), and their combination have an effect on the jawbone in estrogen deficient rats. Six female rats (4-month-old) were used for each sham surgery (SHAM). Twenty-four rats (4-month-old) were ovariectomized and randomly assigned to four equal groups: saline injection (VEH), PTH following saline injection (VEH/PTH), bisphosphonate (ALN), or a combination (ALN/PTH). A hemimandible was randomly dissected from each rat for multiscale (10⁻² to 10⁻⁷ m) characterization including static and dynamic mechanical stability of teeth in the alveolar socket, tissue mineral density distribution (TMD), and nanoindentation properties of the jawbone matrix. Most jawbone characteristics in OVX and its treatment groups were not significantly different from those of the SHAM group. The surface of alveolar bone (AB) surrounding teeth showed a trend of more erosion and addition of new bone tissues in the OVX rat groups compared to the SHAM group. All TMD parameters rapidly increased up to 60 μm from the periodontal ligament surrounding teeth regardless of the treatment groups. Treatments using each therapeutic agent and its combination did not substantially change those characteristics of jawbones in OVX rats. These findings are different from those of lumbar vertebrae in the same rats that showed a significant bone alteration by OVX and treatments. Thus, the current multiscale characterization of jawbone provides comprehensive information that can help better understand jawbone-specific responses to bone-related complications, including postmenopausal osteoporosis and bisphosphonate-related osteonecrosis of the jaw.

查看原文本刊更多论文

骨质疏松治疗剂治疗颌骨的多尺度表征

本研究的目的是确定双膦酸盐（阿仑膦酸钠（ALN））、甲状旁腺激素（PTH）及其联合治疗是否对雌激素缺乏大鼠颌骨有影响。每个假手术（sham）采用6只雌性大鼠（4月龄）。取24只4月龄大鼠切除卵巢，随机分为生理盐水注射组（VEH）、生理盐水注射后甲状旁腺激素组（VEH/PTH）、双膦酸盐组（ALN）、双膦酸盐组（ALN/PTH）。随机从每只大鼠中取出一颗半颌骨进行多尺度（10−2至10−7 m）表征，包括牙槽窝内牙齿的静态和动态力学稳定性、组织矿物质密度分布（TMD）和颌骨基质的纳米压痕特性。OVX组及其治疗组的颌骨特征与SHAM组无显著性差异。与SHAM组相比，OVX组大鼠牙周牙槽骨（AB）表面出现更多糜烂和新骨组织增加的趋势。各治疗组TMD参数均迅速升高至60 μm以内。使用每种治疗剂及其组合治疗并没有实质性地改变OVX大鼠颌骨的这些特征。这些发现不同于同一大鼠的腰椎，显示出OVX和治疗的显著骨改变。因此，目前颌骨的多尺度表征提供了全面的信息，可以帮助更好地了解颌骨对骨相关并发症的特异性反应，包括绝经后骨质疏松症和双磷酸盐相关的颌骨骨坏死。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of the Mechanical Behavior of Biomedical Materials 工程技术-材料科学：生物材料

CiteScore

7.20

自引率

7.70%

发文量

505

审稿时长

46 days

期刊介绍： The Journal of the Mechanical Behavior of Biomedical Materials is concerned with the mechanical deformation, damage and failure under applied forces, of biological material (at the tissue, cellular and molecular levels) and of biomaterials, i.e. those materials which are designed to mimic or replace biological materials. The primary focus of the journal is the synthesis of materials science, biology, and medical and dental science. Reports of fundamental scientific investigations are welcome, as are articles concerned with the practical application of materials in medical devices. Both experimental and theoretical work is of interest; theoretical papers will normally include comparison of predictions with experimental data, though we recognize that this may not always be appropriate. The journal also publishes technical notes concerned with emerging experimental or theoretical techniques, letters to the editor and, by invitation, review articles and papers describing existing techniques for the benefit of an interdisciplinary readership.