Metagenomics reveals the effects of long-term greenhouse vegetable cultivation on soil microbial communities and carbon cycle functions

IF 3.9 2区 农林科学 Q1 AGRONOMY
Xiaoyu Zhang, Yan Yin, Liyu Du, Fengming Xi, Jiaoyue Wang
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Abstract

Background and aims

Soil microbial community structure and diversity are critical for maintaining the health of soil ecosystems. However, how soil microbes regulate the soil carbon cycle under long-term greenhouse cultivation remains unclear.

Methods

We used macro-genomics to analyze changes in microbial community structure and diversity as well as carbon cycle-related functional genes in a long-term greenhouse-grown soil.

Results

Long-term greenhouse cultivation significantly altered soil microbial community structure, manifested by decreased bacterial diversity and increased fungal diversity. The rTCA cycle served as the dominant carbon fixation pathway, with microbial carbon fixation capacity markedly reduced during the initial greenhouse phase but gradually restored as cultivation duration extended. Prolonged greenhouse practices elevated the abundance of degradation genes for labile carbon (i.e. starch) while suppressing those for recalcitrant carbon (i.e. cellulose and lignin). Soil pH is the primary driver of changes in microbial community structure and shifts in carbon cycling functional genes.

Conclusions

Long-term greenhouse cultivation reshaped the microbial community structure by altering soil properties, thereby driving adaptive shifts in microbial carbon cycling functions. The findings provide new insights into the microbial mechanisms underlying soil carbon cycling in long-term greenhouse vegetable cultivation.

宏基因组学揭示了长期温室蔬菜栽培对土壤微生物群落和碳循环功能的影响
背景与目的土壤微生物群落结构和多样性对维持土壤生态系统的健康至关重要。然而,长期温室栽培下土壤微生物如何调节土壤碳循环尚不清楚。方法采用宏观基因组学方法分析长期温室栽培土壤中微生物群落结构、多样性及碳循环相关功能基因的变化。结果长期温室栽培显著改变了土壤微生物群落结构,表现为细菌多样性降低,真菌多样性增加。rTCA循环是主要的固碳途径,微生物固碳能力在温室初始阶段显著降低,但随着栽培时间的延长逐渐恢复。长期温室实践提高了不稳定碳(如淀粉)降解基因的丰度,同时抑制了顽固碳(如纤维素和木质素)的降解基因的丰度。土壤pH值是微生物群落结构变化和碳循环功能基因转移的主要驱动因素。结论长期温室栽培通过改变土壤性质重塑微生物群落结构,从而推动微生物碳循环功能的适应性转变。这些发现为长期温室蔬菜栽培土壤碳循环的微生物机制提供了新的见解。
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来源期刊
Plant and Soil
Plant and Soil 农林科学-农艺学
CiteScore
8.20
自引率
8.20%
发文量
543
审稿时长
2.5 months
期刊介绍: Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.
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