Xiaoyu Zhang, Yan Yin, Liyu Du, Fengming Xi, Jiaoyue Wang
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引用次数: 0
Abstract
Background and aims
Soil microbial community structure and diversity are critical for maintaining the health of soil ecosystems. However, how soil microbes regulate the soil carbon cycle under long-term greenhouse cultivation remains unclear.
Methods
We used macro-genomics to analyze changes in microbial community structure and diversity as well as carbon cycle-related functional genes in a long-term greenhouse-grown soil.
Results
Long-term greenhouse cultivation significantly altered soil microbial community structure, manifested by decreased bacterial diversity and increased fungal diversity. The rTCA cycle served as the dominant carbon fixation pathway, with microbial carbon fixation capacity markedly reduced during the initial greenhouse phase but gradually restored as cultivation duration extended. Prolonged greenhouse practices elevated the abundance of degradation genes for labile carbon (i.e. starch) while suppressing those for recalcitrant carbon (i.e. cellulose and lignin). Soil pH is the primary driver of changes in microbial community structure and shifts in carbon cycling functional genes.
Conclusions
Long-term greenhouse cultivation reshaped the microbial community structure by altering soil properties, thereby driving adaptive shifts in microbial carbon cycling functions. The findings provide new insights into the microbial mechanisms underlying soil carbon cycling in long-term greenhouse vegetable cultivation.
期刊介绍:
Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.