Biodegradation Characterization and Mechanism of Low-Density Polyethylene by the Enriched Mixed-Culture from Plastic-Contaminated Soil

IF 12.2 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials Pub Date : 2025-05-07 DOI:10.1016/j.jhazmat.2025.138530

Zhen Zhang, Xinxin Fan, Rumeng Zhang, Xinghui Pan, Xuexue Zhang, Yi Ding, Ying Liu

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Abstract

Plastic pollution poses significant ecological and health risks. In this study, we enriched microbial consortia from plastic-contaminated soil capable of degrading low-density polyethylene (LDPE) film over a 28-day incubation period. Using two kinds of enriched cultures, the mean film weight loss rate (WLR) of 0.27 ± 0.04% (p < 0.01) was 9 times higher than the control. Scanning electron microscopy (SEM) revealed a average hole occurrence area of 0.67 ± 0.11 μm² in the topmost sample, while the control had no change. Fourier transform infrared (FTIR) revealed specific changes in hydrophilicity (increased by 5.70 ± 0.02 times) and crystallinity (decreased by 15.73 ± 3.26%). Meanwhile, FTIR analyses including peak occurrence at 3,741 cm^-1, carbonyl index and Lambert-Beer law calculations revealed moisture infiltration and predominant aldehyde carbonyl formation (88.69% in total carbonyl). The results of high-throughput sequencing indicated Brevibacillus, Bacillus and Sporosarcina were dominate genera in the mixed-cultures, and PICRUSt2 implied they could use LDPE as the sole carbon source. Our study aims to provided theoretical basis driving plastic degradation and to mitigate plastic pollution based on microbial resource development.

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塑料污染土壤中低密度聚乙烯富集混合培养生物降解特性及机理研究

塑料污染造成严重的生态和健康风险。在这项研究中，我们从塑料污染的土壤中富集了能够在28天的潜伏期内降解低密度聚乙烯（LDPE）薄膜的微生物群落。使用两种富集培养物，平均膜失重率（WLR）为0.27±0.04% (p <；0.01)，是对照组的9倍。扫描电镜（SEM）显示，最上层样品的平均空穴出现面积为0.67±0.11 μm2，而对照组没有变化。傅里叶变换红外（FTIR）显示了亲水性（提高5.70±0.02倍）和结晶度（降低15.73±3.26%）的特殊变化。同时，FTIR分析（包括3741 cm-1处峰值的出现）、羰基指数和Lambert-Beer定律计算表明，水分渗透和主要的醛羰基形成（占总羰基的88.69%）。高通量测序结果显示，短芽孢杆菌（Brevibacillus）、芽孢杆菌（Bacillus）和孢子孢杆菌（Sporosarcina）在混合培养中占优势属，PICRUSt2提示它们可以以LDPE作为唯一碳源。我们的研究旨在为塑料降解提供理论依据，并基于微生物资源的开发来缓解塑料污染。

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来源期刊

Journal of Hazardous Materials 工程技术-工程：环境

CiteScore

25.40

自引率

5.90%

发文量

3059

审稿时长

58 days

期刊介绍： The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.