Diversification of CLE expression patterns and nonmeristematic roles for CLAVATA receptor-like kinases in a moss

IF 8.3 1区 生物学 Q1 PLANT SCIENCES
New Phytologist Pub Date : 2025-05-06 DOI:10.1111/nph.70170
Zoe Nemec-Venza, George R. L. Greiff, C. Jill Harrison
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引用次数: 0

Abstract

CLAVATA受体样激酶在苔藓中CLE表达模式的多样化和非分生组织的作用。
CLAVATA通路控制拟南芥发育过程中分生细胞增殖和多个非分生过程。虽然CLAVATA在非种子植物配子体中可以调节分生组织的增殖,但在陆地植物中孢子体和非分生组织的祖先功能尚不清楚。在这里,我们分析了所有肽(PpCLE)和受体编码(PpCLV1a, PpCLV1b和PpRPK2)基因在苔藓(Physcomitrium patens)生命周期中的启动子活性,并用突变表型数据验证了我们的表达分析。在配子体顶端,PpCLE3表达标记于顶端细胞,PpCLV1b和PpRPK2重叠。在非分生组织中,配子体的PpCLE高度集中,但受体编码基因的表达范围更广,并且许多基因共表达。突变表型分析揭示了PpCLV1a、PpCLV1b和PpRPK2在生育和雌雄生殖发育中的作用。在孢子体中,没有PpCLE表达特异性标记顶端细胞,PpCLV1b和PpRPK2表达最初标记不同的顶端和基部结构域,但后来在层间分生组织重叠。总体而言,孢子体中共表达的基因少于配子体,但所有基因都在保护细胞中共表达。我们的数据表明,CLAVATA在配子囊发育和气孔发育中的非分生功能可能起源于陆地植物。肽编码(CLE)基因拷贝数在苔藓中扩增,启动子进化可能是苔藓进化过程中细胞类型多样化的驱动因素。
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来源期刊
New Phytologist
New Phytologist 生物-植物科学
自引率
5.30%
发文量
728
期刊介绍: New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.
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