Development of a high-performance sliding microneedle-lateral flow immunoassay strip device for ultra-rapid point-of-care diagnosis†

IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2025-05-06 DOI:10.1039/D5LC00199D
Soo-bin Yu and Jae Hwan Jung
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引用次数: 0

Abstract

Interstitial fluid (ISF) is a promising biofluid for non-invasive diagnostics, but its clinical application is limited by slow extraction rates and small sample volumes. To address these challenges, we developed the high-performance sliding microneedle-lateral flow immunoassay (HP-SML) device, an improved microneedle (MN)-based platform for rapid ISF extraction and on-site biomarker detection. The device incorporates extended MNs (1400 μm), a 3D-printed insertion case, and a lateral flow assay (LFA), enabling efficient ISF collection and immediate visual confirmation of test results. The HP-SML device demonstrated a 5-fold increase in ISF extraction speed compared to conventional SML devices, achieving 1.5 μL min−1 extraction rates with minimal tissue disruption and rapid skin recovery within 15 minutes. The device successfully detected C-reactive protein (CRP) at a clinically relevant limit of 104 ng mL−1, confirming its applicability for Hodgkin lymphoma (HL) prognosis monitoring. It also exhibited high specificity for CRP, with no cross-reactivity observed against other inflammatory biomarkers such as IL-6, TNF-α, MMP-9, and MMP-2. Furthermore, ISF-derived CRP levels strongly correlated with serum CRP concentrations, validating ISF as a viable alternative for HL management. Given its high efficiency, ease of use, and on-site detection capabilities, the HP-SML device presents a significant advancement in point-of-care diagnostics and continuous disease monitoring.

一种用于超快速即时诊断的高性能滑动微针横向流动免疫测定条带装置的开发。
间质液(ISF)是一种很有前途的非侵入性诊断生物液,但其临床应用受到提取速度慢和样本量小的限制。为了应对这些挑战,我们开发了高性能滑动微针横向流动免疫测定(HP-SML)装置,这是一种基于微针(MN)的改进平台,用于快速提取ISF和现场生物标志物检测。该设备集成了扩展的MNs (1400 μm)、3d打印插入盒和横向流动分析(LFA),可实现高效的ISF收集和测试结果的即时视觉确认。HP-SML设备与传统SML设备相比,ISF提取速度提高了5倍,在15分钟内实现1.5 μL min-1的提取速率,组织破坏最小,皮肤恢复迅速。该装置成功检测c反应蛋白(CRP),临床相关限为104 ng mL-1,证实了其在霍奇金淋巴瘤(HL)预后监测中的适用性。它对CRP也有很高的特异性,对其他炎症生物标志物如IL-6、TNF-α、MMP-9和MMP-2没有交叉反应。此外,ISF衍生的CRP水平与血清CRP浓度密切相关,验证了ISF作为HL治疗的可行替代方案。鉴于其高效率、易用性和现场检测能力,HP-SML设备在即时诊断和连续疾病监测方面取得了重大进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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