Integrating Intestinal Biotransformation and Gut Microbiota to Uncover the Influence of Tongfu Xiexia Decoction in Rats With Constipation

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Rapid Communications in Mass Spectrometry Pub Date : 2025-05-06 DOI:10.1002/rcm.10065

Yu-Han Piao, Jing Luo, Tao Ren, Ao Wang, Yu-Yun Li, Li-Jia Pan, Xin-Ze Li, Fang-Tong Li, Yu-Wen Bao, Fei Zheng, Hao Yue

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引用次数: 0

Abstract

Rationale

Tongfu Xiexia Decoction (TFXXD), a traditional Chinese medicine formula, comprises six herbs: semen raphani, Rehmannia glutinosa, rhubarb, Magnolia officinalis, trifoliate orange, and mirabilite. The aim of this study was to investigate the effects of TFXXD on its biotransformation and microbial abundance in the gut of constipated rats.

Methods

Ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to analyze the biotransformation of TFXXD in the colonic contents, and 16S rRNA sequencing was used to assess the structure and diversity of the gut microbiota across various rat groups.

Results

We identified and analyzed 25 biotransformations of TFXXD in the colonic contents, which undergo various reactions such as deglycosylation, ring opening, and hydration in intestinal bacteria, with 14 originating from trifoliate orange, six from rhubarb, three from Rehmannia glutinosa, one from Semen raphani, and one from M. officinalis. 16S rRNA analysis revealed that TFXXD significantly enhanced the relative abundance of beneficial bacteria, such as Lactobacillus and Bacteroides while significantly reducing Oscillospira abundance. Moreover, TFXXD considerably affected the carbohydrate and amino acid metabolism. Correlation analyses revealed a significant negative correlation between Bacteroides and ACH (Acetylcholine), NO (nitric oxide), D-Lac (D-Lactate), IL-6 (Interleukin-6), TNF-α (tumor necrosis factor-α), and IL-1β (Interleukin-1β) and a significant positive correlation between Bacteroides, hesperetin, and rhein.

Conclusions

In conclusion, our findings indicate that TFXXD can modulate the structure and diversity of the gut microbiota and enhance the metabolic balance in constipated rats.

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结合肠道生物转化和肠道菌群揭示通腑泻泻汤对便秘大鼠的影响

通腑泻泻汤（TFXXD）是一种中药配方，由六种草药组成：大麻仁、地黄、大黄、厚朴、三叶橙和芒萁。本研究旨在探讨TFXXD对便秘大鼠肠道生物转化和微生物丰度的影响。方法采用超高效液相色谱-四极杆飞行时间质谱（UHPLC-Q-TOF-MS/MS）技术分析TFXXD在大肠内容物中的生物转化，并采用16S rRNA测序技术评估各组大鼠肠道微生物群的结构和多样性。结果在大肠内容物中鉴定并分析了25种TFXXD的生物转化，其中14种来自三叶橙，6种来自大黄，3种来自地黄，1种来自raphani， 1种来自M. officinalis。16S rRNA分析显示，TFXXD显著提高了乳酸菌和拟杆菌等有益菌的相对丰度，同时显著降低了Oscillospira的丰度。此外，TFXXD显著影响了碳水化合物和氨基酸的代谢。相关分析显示拟杆菌与乙酰胆碱（ACH）、一氧化氮（NO）、乳酸D-Lac、白细胞介素-6 （IL-6）、肿瘤坏死因子-α (TNF-α)、白细胞介素-1β (IL-1β)呈显著负相关，拟杆菌与橙皮苷、大黄素呈显著正相关。结论TFXXD可调节便秘大鼠肠道菌群结构和多样性，促进代谢平衡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.