Three fluorescent probes based on the D-π-A configuration to detect changes in endoplasmic reticulum polarity

IF 4.1 3区 化学 Q2 CHEMISTRY, PHYSICAL
Jie Wang , Sichen Zhang , Jiale Li , Aobo Sun , Aixiang Ding , Lei Hu , Chunfei Wang , Hui Wang
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Abstract

Abnormal fluctuations in the polarity of the endoplasmic reticulum (ER), a critical intracellular organelle, have been closely associated with the onset and progression of various diseases. Consequently, monitoring ER polarity fluctuations is of significant practical importance for the diagnosis and treatment of these conditions. In this study, we designed and synthesized three ER-targeted polarity fluorescent probes, TCF1-TCF3, based on a D-π-A configuration, using the tricyanofuran as the parent material. These probes demonstrated a robust response to polarity changes, enabling quantitative monitoring of environmental polarity across a broad range. Furthermore, TCF1-TCF3 exhibited high selectivity, excellent photostability, and minimal interference from pH variations, environmental analytes and viscosity. Cell imaging experiments revealed that TCF1-TCF3 possessed low cytotoxicity, effectively target the ER, and exhibited superior imaging capabilities. Notably, the exemplary probe TCF3 achieved high-quality imaging even at a low concentration of 0.5 μM. These properties enable TCF3 to effectively monitor ER polarity changes induced by a variety of conditions such as DTT, tunicamycin, etc., highlighting its potential as a valuable tool for studying ER-related cellular processes.

Abstract Image

基于D-π-A结构的三种荧光探针检测内质网极性的变化
内质网(ER)是细胞内重要的细胞器,其极性的异常波动与多种疾病的发生和发展密切相关。因此,监测内质网极性波动对这些疾病的诊断和治疗具有重要的实际意义。本研究以三氰呋喃为母体材料,设计合成了3个基于D-π-A构型的er定向极性荧光探针TCF1-TCF3。这些探针显示出对极性变化的强大响应,能够在广泛的范围内对环境极性进行定量监测。此外,TCF1-TCF3表现出高选择性、优异的光稳定性,并且受pH变化、环境分析物和粘度的干扰最小。细胞成像实验显示,TCF1-TCF3具有较低的细胞毒性,能有效靶向内质网,具有较好的成像能力。值得注意的是,示例性探针TCF3即使在0.5 μM的低浓度下也能实现高质量的成像。这些特性使TCF3能够有效地监测各种条件(如DTT、tunicamycin等)诱导的内质网极性变化,突出了其作为研究内质网相关细胞过程的有价值工具的潜力。
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来源期刊
CiteScore
7.90
自引率
7.00%
发文量
580
审稿时长
48 days
期刊介绍: JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds. All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor). The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.
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