Jie Wang , Sichen Zhang , Jiale Li , Aobo Sun , Aixiang Ding , Lei Hu , Chunfei Wang , Hui Wang
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引用次数: 0
Abstract
Abnormal fluctuations in the polarity of the endoplasmic reticulum (ER), a critical intracellular organelle, have been closely associated with the onset and progression of various diseases. Consequently, monitoring ER polarity fluctuations is of significant practical importance for the diagnosis and treatment of these conditions. In this study, we designed and synthesized three ER-targeted polarity fluorescent probes, TCF1-TCF3, based on a D-π-A configuration, using the tricyanofuran as the parent material. These probes demonstrated a robust response to polarity changes, enabling quantitative monitoring of environmental polarity across a broad range. Furthermore, TCF1-TCF3 exhibited high selectivity, excellent photostability, and minimal interference from pH variations, environmental analytes and viscosity. Cell imaging experiments revealed that TCF1-TCF3 possessed low cytotoxicity, effectively target the ER, and exhibited superior imaging capabilities. Notably, the exemplary probe TCF3 achieved high-quality imaging even at a low concentration of 0.5 μM. These properties enable TCF3 to effectively monitor ER polarity changes induced by a variety of conditions such as DTT, tunicamycin, etc., highlighting its potential as a valuable tool for studying ER-related cellular processes.
期刊介绍:
JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds.
All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor).
The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.