Rhizosphere as hotspot for ammonia oxidation in secondary effluent constructed wetlands: Role of comammox Nitrospira

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-05-02 DOI:10.1016/j.biortech.2025.132621

Yize Zheng, Bowen Wang, Hongwei Chen, Jinyang Zhou, Chao Song, Jin Chen, Zimin Chai, Maosheng Zheng

{"title":"Rhizosphere as hotspot for ammonia oxidation in secondary effluent constructed wetlands: Role of comammox Nitrospira","authors":"Yize Zheng, Bowen Wang, Hongwei Chen, Jinyang Zhou, Chao Song, Jin Chen, Zimin Chai, Maosheng Zheng","doi":"10.1016/j.biortech.2025.132621","DOIUrl":null,"url":null,"abstract":"<div><div>Secondary effluent constructed wetlands (SECWs) are engineered ecosystems for advanced wastewater treatment, yet the functional roles and survival strategies of complete ammonia oxidizers (comammox) within these systems remain poorly understood, particularly from a rhizosphere view. The results of this study demonstrated that comammox was numerically and functionally dominant (60.4 % to 70.6 %) in SECWs. The rhizosphere acted as a hotspot for ammonia oxidation and N<sub>2</sub>O production, compared to the nonrhizosphere. Enhanced nitrification was attributed to radial oxygen loss and humic acid-like compounds in root exudates. Furthermore, variations in comammox community structure and ammonia kinetic properties (K<sub>m(app)</sub> = 0.140 ± 0.026 mg N L<sup>−1</sup>) revealed niche differentiation among comammox species: the r-strategist <em>Nitrospira</em> sp. HN-bin3 thrived over time, whereas the K-strategist <em>Nitrospira nitrificans</em> was outcompeted, with ammonia concentration identified as the main driving factor. These results highlighted the vital but underappreciated role of comammox in the nitrogen cycle of constructed wetlands and provided new insights into their ecological functions and adaptive strategies.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132621"},"PeriodicalIF":9.7000,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresource Technology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0960852425005875","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURAL ENGINEERING","Score":null,"Total":0}

引用次数: 0

Abstract

Secondary effluent constructed wetlands (SECWs) are engineered ecosystems for advanced wastewater treatment, yet the functional roles and survival strategies of complete ammonia oxidizers (comammox) within these systems remain poorly understood, particularly from a rhizosphere view. The results of this study demonstrated that comammox was numerically and functionally dominant (60.4 % to 70.6 %) in SECWs. The rhizosphere acted as a hotspot for ammonia oxidation and N₂O production, compared to the nonrhizosphere. Enhanced nitrification was attributed to radial oxygen loss and humic acid-like compounds in root exudates. Furthermore, variations in comammox community structure and ammonia kinetic properties (K_m(app) = 0.140 ± 0.026 mg N L⁻¹) revealed niche differentiation among comammox species: the r-strategist Nitrospira sp. HN-bin3 thrived over time, whereas the K-strategist Nitrospira nitrificans was outcompeted, with ammonia concentration identified as the main driving factor. These results highlighted the vital but underappreciated role of comammox in the nitrogen cycle of constructed wetlands and provided new insights into their ecological functions and adaptive strategies.

Abstract Image

查看原文本刊更多论文

根际作为二级出水人工湿地氨氧化的热点：硝化毛菌的作用

二级出水人工湿地（SECWs）是用于高级废水处理的工程生态系统，但这些系统中完全氨氧化剂（comammox）的功能作用和生存策略仍然知之甚少，特别是从根际的角度来看。本研究结果表明，comammox在secw中在数量和功能上占主导地位（60.4%至70.6%）。与非根际相比，根际是氨氧化和N2O生产的热点。强化硝化作用归因于径向氧损失和根分泌物中的腐植酸类化合物。此外，群落结构和氨动力学性质（Km(app) = 0.140±0.026 mg N L−1）的变化揭示了不同群落间的生态位分化：随着时间的推移，r-战略型硝化螺旋菌（N-bin3）在生态环境中处于优势地位，而k -战略型硝化螺旋菌（k -战略型硝化螺旋菌）则处于优势地位，氨浓度是主要驱动因素。这些结果突出了comammox在人工湿地氮循环中的重要作用，并为其生态功能和适应策略提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.